MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1356 367.1751 366.1678
1357 367.1752 366.1679
1358 367.1753 366.1680
1359 367.1754 366.1681
1360 367.1756 366.1683
1361 367.1757 366.1684
1362 367.1758 366.1685
1363 367.1921 366.1848
1364 367.2095 366.2022
1365 367.2115 366.2042
1366 367.2120 366.2047
1367 367.2121 366.2048
1368 367.2124 366.2051
1369 367.2322 366.2249
1370 367.2325 366.2252
1371 367.2325 366.2252
1372 367.2328 366.2255
1373 367.2351 366.2278
1374 367.2466 366.2393
1375 368.2310 367.2237
1376 369.0881 368.0808
1377 369.0888 368.0815
1378 369.1183 368.1110
1379 369.1230 368.1157
1380 369.1333 368.1260
1381 369.1410 368.1337
1382 369.1527 368.1454
1383 369.1531 368.1458
1384 369.1534 368.1461
1385 369.1536 368.1463
1386 369.1539 368.1466
1387 369.1542 368.1469
1388 369.1542 368.1469
1389 369.1545 368.1472
1390 369.1546 368.1473
1391 369.1896 368.1823
1392 369.1898 368.1825
1393 369.1904 368.1831
1394 369.1907 368.1834
1395 369.1907 368.1834
1396 369.1908 368.1835
1397 369.1910 368.1837
1398 369.1910 368.1837
1399 369.1911 368.1838
1400 369.1912 368.1839
1401 369.1912 368.1839
1402 369.2094 368.2021
1403 369.2095 368.2022
1404 369.2118 368.2045
1405 369.2258 368.2185
1406 369.2264 368.2191
1407 369.2266 368.2193
1408 369.2273 368.2200
1409 369.2274 368.2201
1410 369.2274 368.2201
1411 369.2275 368.2202
1412 369.2276 368.2203
1413 369.2277 368.2204
1414 369.2280 368.2207
1415 369.2479 368.2406
1416 369.2481 368.2408
1417 369.2481 368.2408
1418 369.2484 368.2411
1419 371.0650 370.0577
1420 371.1031 370.0958
1421 371.1034 370.0961
1422 371.1035 370.0962
1423 371.1039 370.0966
1424 371.1206 370.1133
1425 371.1391 370.1318
1426 371.1573 370.1500
1427 371.1694 370.1621
1428 371.1709 370.1636
1429 371.2413 370.2340
1430 371.2423 370.2350
1431 371.3165 370.3092
1432 371.3166 370.3093
1433 371.3167 370.3094
1434 371.3168 370.3095
1435 371.3168 370.3095
1436 371.3169 370.3096
1437 371.3170 370.3097
1438 371.3170 370.3097
1439 371.3170 370.3097
1440 371.3171 370.3098
1441 371.3171 370.3098
1442 371.3172 370.3099
1443 371.3172 370.3099
1444 371.3172 370.3099
1445 371.3174 370.3101
1446 371.3175 370.3102
1447 371.3175 370.3102
1448 371.3176 370.3103
1449 371.3177 370.3104
1450 371.3178 370.3105
1451 371.3179 370.3106
1452 371.3179 370.3106
1453 371.3179 370.3106
1454 371.3180 370.3107
1455 371.3180 370.3107
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