MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1256 363.1784 362.1711
1257 363.1787 362.1714
1258 363.1790 362.1717
1259 363.1790 362.1717
1260 363.1792 362.1719
1261 363.1794 362.1721
1262 363.1800 362.1727
1263 363.1800 362.1727
1264 363.1801 362.1728
1265 363.1801 362.1728
1266 363.1803 362.1730
1267 363.1803 362.1730
1268 363.1803 362.1730
1269 363.1804 362.1731
1270 363.1805 362.1732
1271 363.1805 362.1732
1272 363.1805 362.1732
1273 363.1806 362.1733
1274 363.1807 362.1734
1275 363.1808 362.1735
1276 363.2173 362.2100
1277 364.2346 363.2273
1278 365.0314 364.0241
1279 365.0328 364.0255
1280 365.0840 364.0767
1281 365.1212 364.1139
1282 365.1221 364.1148
1283 365.1222 364.1149
1284 365.1223 364.1150
1285 365.1231 364.1158
1286 365.1580 364.1507
1287 365.1581 364.1508
1288 365.1581 364.1508
1289 365.1582 364.1509
1290 365.1584 364.1511
1291 365.1586 364.1513
1292 365.1586 364.1513
1293 365.1587 364.1514
1294 365.1588 364.1515
1295 365.1594 364.1521
1296 365.1595 364.1522
1297 365.1596 364.1523
1298 365.1596 364.1523
1299 365.1596 364.1523
1300 365.1599 364.1526
1301 365.1600 364.1527
1302 365.1601 364.1528
1303 365.1604 364.1531
1304 365.1944 364.1871
1305 365.1948 364.1875
1306 365.1949 364.1876
1307 365.1951 364.1878
1308 365.1952 364.1879
1309 365.1953 364.1880
1310 365.1954 364.1881
1311 365.1956 364.1883
1312 365.1958 364.1885
1313 365.1959 364.1886
1314 365.1959 364.1886
1315 365.1960 364.1887
1316 365.1960 364.1887
1317 365.1960 364.1887
1318 365.1962 364.1889
1319 365.1962 364.1889
1320 365.1962 364.1889
1321 365.1964 364.1891
1322 365.1969 364.1896
1323 365.2154 364.2081
1324 365.2155 364.2082
1325 365.2159 364.2086
1326 365.2159 364.2086
1327 367.0478 366.0405
1328 367.0727 366.0654
1329 367.0739 366.0666
1330 367.1368 366.1295
1331 367.1374 366.1301
1332 367.1374 366.1301
1333 367.1378 366.1305
1334 367.1378 366.1305
1335 367.1378 366.1305
1336 367.1380 366.1307
1337 367.1384 366.1311
1338 367.1385 366.1312
1339 367.1385 366.1312
1340 367.1388 366.1315
1341 367.1389 366.1316
1342 367.1738 366.1665
1343 367.1740 366.1667
1344 367.1742 366.1669
1345 367.1743 366.1670
1346 367.1743 366.1670
1347 367.1743 366.1670
1348 367.1743 366.1670
1349 367.1744 366.1671
1350 367.1745 366.1672
1351 367.1745 366.1672
1352 367.1748 366.1675
1353 367.1750 366.1677
1354 367.1750 366.1677
1355 367.1750 366.1677
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