MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1156 355.1360 354.1287
1157 355.1369 354.1296
1158 355.1374 354.1301
1159 355.1378 354.1305
1160 355.1381 354.1308
1161 355.1382 354.1309
1162 355.1383 354.1310
1163 355.1384 354.1311
1164 355.1387 354.1314
1165 355.1389 354.1316
1166 355.1733 354.1660
1167 355.1736 354.1663
1168 355.1742 354.1669
1169 355.1743 354.1670
1170 355.1748 354.1675
1171 355.1751 354.1678
1172 355.1752 354.1679
1173 355.1933 354.1860
1174 355.1935 354.1862
1175 355.1942 354.1869
1176 355.2691 354.2618
1177 356.1790 355.1717
1178 356.2820 355.2747
1179 356.2820 355.2747
1180 356.2823 355.2750
1181 356.2827 355.2754
1182 357.0857 356.0784
1183 357.0865 356.0792
1184 357.0871 356.0798
1185 357.0873 356.0800
1186 357.0875 356.0802
1187 357.0876 356.0803
1188 357.1257 356.1184
1189 357.1258 356.1185
1190 357.1526 356.1453
1191 357.1534 356.1461
1192 357.1538 356.1465
1193 357.1541 356.1468
1194 357.1543 356.1470
1195 357.1546 356.1473
1196 357.1899 356.1826
1197 357.1903 356.1830
1198 357.2266 356.2193
1199 359.0214 358.0141
1200 359.0662 358.0589
1201 359.1038 358.0965
1202 359.1113 358.1040
1203 359.1124 358.1051
1204 359.1138 358.1065
1205 359.1476 358.1403
1206 359.1490 358.1417
1207 359.1704 358.1631
1208 359.2050 358.1977
1209 359.2056 358.1983
1210 359.2411 358.2338
1211 361.0899 360.0826
1212 361.1271 360.1198
1213 361.1274 360.1201
1214 361.1281 360.1208
1215 361.1632 360.1559
1216 361.1635 360.1562
1217 361.1636 360.1563
1218 361.1636 360.1563
1219 361.1645 360.1572
1220 361.1645 360.1572
1221 361.1646 360.1573
1222 361.1646 360.1573
1223 361.1646 360.1573
1224 361.1647 360.1574
1225 361.1647 360.1574
1226 361.1647 360.1574
1227 361.1650 360.1577
1228 361.1650 360.1577
1229 361.2005 360.1932
1230 361.2009 360.1936
1231 361.2010 360.1937
1232 361.2011 360.1938
1233 361.2014 360.1941
1234 361.2455 360.2382
1235 361.2455 360.2382
1236 361.2462 360.2389
1237 361.2469 360.2396
1238 363.0534 362.0461
1239 363.1045 362.0972
1240 363.1051 362.0978
1241 363.1425 362.1352
1242 363.1426 362.1353
1243 363.1426 362.1353
1244 363.1428 362.1355
1245 363.1429 362.1356
1246 363.1430 362.1357
1247 363.1431 362.1358
1248 363.1433 362.1360
1249 363.1437 362.1364
1250 363.1438 362.1365
1251 363.1438 362.1365
1252 363.1439 362.1366
1253 363.1441 362.1368
1254 363.1441 362.1368
1255 363.1778 362.1705
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