MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1056 349.2214 348.2141
1057 349.2218 348.2145
1058 349.2224 348.2151
1059 351.0501 350.0428
1060 351.0777 350.0704
1061 351.1422 350.1349
1062 351.1423 350.1350
1063 351.1423 350.1350
1064 351.1424 350.1351
1065 351.1425 350.1352
1066 351.1426 350.1353
1067 351.1428 350.1355
1068 351.1428 350.1355
1069 351.1432 350.1359
1070 351.1432 350.1359
1071 351.1435 350.1362
1072 351.1435 350.1362
1073 351.1436 350.1363
1074 351.1436 350.1363
1075 351.1436 350.1363
1076 351.1789 350.1716
1077 351.1790 350.1717
1078 351.1790 350.1717
1079 351.1790 350.1717
1080 351.1792 350.1719
1081 351.1792 350.1719
1082 351.1793 350.1720
1083 351.1793 350.1720
1084 351.1795 350.1722
1085 351.1797 350.1724
1086 351.1797 350.1724
1087 351.1797 350.1724
1088 351.1799 350.1726
1089 351.1800 350.1727
1090 351.1801 350.1728
1091 351.1802 350.1729
1092 351.1803 350.1730
1093 351.1803 350.1730
1094 351.1804 350.1731
1095 351.1804 350.1731
1096 351.1805 350.1732
1097 351.1807 350.1734
1098 351.1989 350.1916
1099 351.1992 350.1919
1100 351.2153 350.2080
1101 351.2153 350.2080
1102 351.2158 350.2085
1103 351.2159 350.2086
1104 351.2161 350.2088
1105 351.2165 350.2092
1106 351.2167 350.2094
1107 351.2167 350.2094
1108 351.2168 350.2095
1109 351.2172 350.2099
1110 351.2363 350.2290
1111 351.2363 350.2290
1112 351.2367 350.2294
1113 351.2369 350.2296
1114 351.2371 350.2298
1115 353.0936 352.0863
1116 353.0938 352.0865
1117 353.1097 352.1024
1118 353.1208 352.1135
1119 353.1212 352.1139
1120 353.1213 352.1140
1121 353.1214 352.1141
1122 353.1217 352.1144
1123 353.1219 352.1146
1124 353.1222 352.1149
1125 353.1223 352.1150
1126 353.1228 352.1155
1127 353.1578 352.1505
1128 353.1581 352.1508
1129 353.1582 352.1509
1130 353.1582 352.1509
1131 353.1583 352.1510
1132 353.1584 352.1511
1133 353.1584 352.1511
1134 353.1585 352.1512
1135 353.1586 352.1513
1136 353.1586 352.1513
1137 353.1592 352.1519
1138 353.1594 352.1521
1139 353.1595 352.1522
1140 353.1598 352.1525
1141 353.1598 352.1525
1142 353.1599 352.1526
1143 353.1947 352.1874
1144 353.1955 352.1882
1145 353.2323 352.2250
1146 353.2529 352.2456
1147 353.2530 352.2457
1148 354.2360 353.2287
1149 355.0113 354.0040
1150 355.0717 354.0644
1151 355.0718 354.0645
1152 355.1069 354.0996
1153 355.1080 354.1007
1154 355.1082 354.1009
1155 355.1096 354.1023
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