MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
956 345.1330 344.1257
957 345.1695 344.1622
958 345.1695 344.1622
959 345.1699 344.1626
960 345.1702 344.1629
961 345.4384 344.4311
962 345.6893 344.6820
963 346.2237 345.2164
964 346.2238 345.2165
965 346.2246 345.2173
966 346.2248 345.2175
967 347.1111 346.1038
968 347.1119 346.1046
969 347.1475 346.1402
970 347.1475 346.1402
971 347.1476 346.1403
972 347.1477 346.1404
973 347.1478 346.1405
974 347.1479 346.1406
975 347.1480 346.1407
976 347.1486 346.1413
977 347.1487 346.1414
978 347.1487 346.1414
979 347.1488 346.1415
980 347.1489 346.1416
981 347.1489 346.1416
982 347.1492 346.1419
983 347.1838 346.1765
984 347.1839 346.1766
985 347.1840 346.1767
986 347.1842 346.1769
987 347.1843 346.1770
988 347.1845 346.1772
989 347.1850 346.1777
990 347.1851 346.1778
991 347.1851 346.1778
992 347.1852 346.1779
993 347.1852 346.1779
994 347.1852 346.1779
995 347.1852 346.1779
996 347.1853 346.1780
997 347.1853 346.1780
998 347.1854 346.1781
999 347.1855 346.1782
1000 347.1860 346.1787
1001 347.2300 346.2227
1002 348.2032 347.1959
1003 349.0315 348.0242
1004 349.1255 348.1182
1005 349.1263 348.1190
1006 349.1272 348.1199
1007 349.1272 348.1199
1008 349.1272 348.1199
1009 349.1273 348.1200
1010 349.1274 348.1201
1011 349.1276 348.1203
1012 349.1278 348.1205
1013 349.1279 348.1206
1014 349.1279 348.1206
1015 349.1631 348.1558
1016 349.1632 348.1559
1017 349.1633 348.1560
1018 349.1633 348.1560
1019 349.1634 348.1561
1020 349.1635 348.1562
1021 349.1635 348.1562
1022 349.1636 348.1563
1023 349.1636 348.1563
1024 349.1637 348.1564
1025 349.1637 348.1564
1026 349.1644 348.1571
1027 349.1645 348.1572
1028 349.1645 348.1572
1029 349.1645 348.1572
1030 349.1646 348.1573
1031 349.1646 348.1573
1032 349.1646 348.1573
1033 349.1647 348.1574
1034 349.1647 348.1574
1035 349.1647 348.1574
1036 349.1652 348.1579
1037 349.1851 348.1778
1038 349.1994 348.1921
1039 349.1998 348.1925
1040 349.2000 348.1927
1041 349.2002 348.1929
1042 349.2003 348.1930
1043 349.2003 348.1930
1044 349.2006 348.1933
1045 349.2007 348.1934
1046 349.2009 348.1936
1047 349.2009 348.1936
1048 349.2010 348.1937
1049 349.2010 348.1937
1050 349.2010 348.1937
1051 349.2011 348.1938
1052 349.2012 348.1939
1053 349.2016 348.1943
1054 349.2209 348.2136
1055 349.2211 348.2138
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