MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
856 339.1053 338.0980
857 339.1060 338.0987
858 339.1067 338.0994
859 339.1072 338.0999
860 339.1131 338.1058
861 339.1133 338.1060
862 339.1135 338.1062
863 339.1217 338.1144
864 339.1226 338.1153
865 339.1419 338.1346
866 339.1420 338.1347
867 339.1421 338.1348
868 339.1424 338.1351
869 339.1424 338.1351
870 339.1425 338.1352
871 339.1425 338.1352
872 339.1427 338.1354
873 339.1427 338.1354
874 339.1428 338.1355
875 339.1428 338.1355
876 339.1428 338.1355
877 339.1429 338.1356
878 339.1429 338.1356
879 339.1429 338.1356
880 339.1431 338.1358
881 339.1432 338.1359
882 339.1435 338.1362
883 339.1437 338.1364
884 339.1438 338.1365
885 339.1439 338.1366
886 339.1680 338.1607
887 339.1791 338.1718
888 339.1795 338.1722
889 339.1795 338.1722
890 339.1796 338.1723
891 339.1798 338.1725
892 339.1800 338.1727
893 339.1802 338.1729
894 339.1989 338.1916
895 339.1989 338.1916
896 339.1991 338.1918
897 339.2737 338.2664
898 341.0556 340.0483
899 341.0913 340.0840
900 341.0919 340.0846
901 341.0921 340.0848
902 341.0933 340.0860
903 341.1099 340.1026
904 341.1100 340.1027
905 341.1208 340.1135
906 341.1213 340.1140
907 341.1219 340.1146
908 341.1219 340.1146
909 341.1221 340.1148
910 341.1224 340.1151
911 341.1228 340.1155
912 341.1231 340.1158
913 341.1577 340.1504
914 341.1580 340.1507
915 341.1581 340.1508
916 341.1584 340.1511
917 341.1584 340.1511
918 341.1584 340.1511
919 341.1585 340.1512
920 341.1586 340.1513
921 341.1586 340.1513
922 341.1590 340.1517
923 341.1591 340.1518
924 341.1592 340.1519
925 341.1592 340.1519
926 341.1593 340.1520
927 341.1952 340.1879
928 341.1959 340.1886
929 341.1960 340.1887
930 341.1961 340.1888
931 341.1961 340.1888
932 341.1963 340.1890
933 341.1964 340.1891
934 341.1966 340.1893
935 341.2167 340.2094
936 342.1928 341.1855
937 343.0705 342.0632
938 343.0711 342.0638
939 343.0716 342.0643
940 343.1091 342.1018
941 343.1741 342.1668
942 343.1742 342.1669
943 343.1745 342.1672
944 343.1748 342.1675
945 343.1933 342.1860
946 344.1990 343.1917
947 344.2081 343.2008
948 344.2088 343.2015
949 344.2089 343.2016
950 345.0504 344.0431
951 345.0857 344.0784
952 345.0877 344.0804
953 345.1320 344.1247
954 345.1322 344.1249
955 345.1328 344.1255
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