MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
756 335.1486 334.1413
757 335.1487 334.1414
758 335.1489 334.1416
759 335.1490 334.1417
760 335.1841 334.1768
761 335.1843 334.1770
762 335.1844 334.1771
763 335.1845 334.1772
764 335.1845 334.1772
765 335.1847 334.1774
766 335.1849 334.1776
767 335.1852 334.1779
768 335.1852 334.1779
769 335.1852 334.1779
770 335.1852 334.1779
771 335.1853 334.1780
772 335.1853 334.1780
773 335.1854 334.1781
774 335.1856 334.1783
775 335.1858 334.1785
776 335.2203 334.2130
777 335.2203 334.2130
778 335.2207 334.2134
779 335.2209 334.2136
780 335.2211 334.2138
781 335.2213 334.2140
782 335.2214 334.2141
783 335.2215 334.2142
784 335.2215 334.2142
785 335.2215 334.2142
786 335.2215 334.2142
787 335.2217 334.2144
788 335.2221 334.2148
789 335.2426 334.2353
790 335.9292 334.9219
791 335.9294 334.9221
792 335.9294 334.9221
793 336.2249 335.2176
794 336.8284 335.8211
795 337.0339 336.0266
796 337.0341 336.0268
797 337.0889 336.0816
798 337.0984 336.0911
799 337.1079 336.1006
800 337.1252 336.1179
801 337.1265 336.1192
802 337.1266 336.1193
803 337.1267 336.1194
804 337.1270 336.1197
805 337.1270 336.1197
806 337.1271 336.1198
807 337.1272 336.1199
808 337.1275 336.1202
809 337.1276 336.1203
810 337.1279 336.1206
811 337.1281 336.1208
812 337.1284 336.1211
813 337.1628 336.1555
814 337.1631 336.1558
815 337.1631 336.1558
816 337.1633 336.1560
817 337.1633 336.1560
818 337.1634 336.1561
819 337.1634 336.1561
820 337.1635 336.1562
821 337.1636 336.1563
822 337.1636 336.1563
823 337.1637 336.1564
824 337.1637 336.1564
825 337.1638 336.1565
826 337.1640 336.1567
827 337.1642 336.1569
828 337.1643 336.1570
829 337.1643 336.1570
830 337.1643 336.1570
831 337.1643 336.1570
832 337.1644 336.1571
833 337.1646 336.1573
834 337.1647 336.1574
835 337.1647 336.1574
836 337.1814 336.1741
837 337.1829 336.1756
838 337.1833 336.1760
839 337.1835 336.1762
840 337.2012 336.1939
841 337.2013 336.1940
842 337.2378 336.2305
843 337.2571 336.2498
844 338.1512 337.1439
845 338.1839 337.1766
846 338.2409 337.2336
847 338.6765 337.6692
848 339.0159 338.0086
849 339.0764 338.0691
850 339.0771 338.0698
851 339.0772 338.0699
852 339.0939 338.0866
853 339.1045 338.0972
854 339.1046 338.0973
855 339.1052 338.0979
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