MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
656 331.0715 330.0642
657 331.1162 330.1089
658 331.1164 330.1091
659 331.1534 330.1461
660 331.1535 330.1462
661 331.1537 330.1464
662 331.1537 330.1464
663 331.1538 330.1465
664 331.1539 330.1466
665 331.1540 330.1467
666 331.1769 330.1696
667 331.1898 330.1825
668 331.1901 330.1828
669 331.1902 330.1829
670 331.1904 330.1831
671 331.1906 330.1833
672 332.0575 331.0502
673 332.2087 331.2014
674 332.2088 331.2015
675 333.0564 332.0491
676 333.0571 332.0498
677 333.0573 332.0500
678 333.0875 332.0802
679 333.0939 332.0866
680 333.0943 332.0870
681 333.0949 332.0876
682 333.1319 332.1246
683 333.1320 332.1247
684 333.1320 332.1247
685 333.1320 332.1247
686 333.1321 332.1248
687 333.1322 332.1249
688 333.1323 332.1250
689 333.1328 332.1255
690 333.1329 332.1256
691 333.1329 332.1256
692 333.1329 332.1256
693 333.1552 332.1479
694 333.1685 332.1612
695 333.1685 332.1612
696 333.1687 332.1614
697 333.1687 332.1614
698 333.1687 332.1614
699 333.1688 332.1615
700 333.1692 332.1619
701 333.1693 332.1620
702 333.1693 332.1620
703 333.1695 332.1622
704 333.1696 332.1623
705 333.1698 332.1625
706 333.1699 332.1626
707 333.1700 332.1627
708 333.1702 332.1629
709 333.1703 332.1630
710 333.1916 332.1843
711 333.2045 332.1972
712 333.2047 332.1974
713 333.2050 332.1977
714 333.2054 332.1981
715 333.2056 332.1983
716 333.2056 332.1983
717 333.2057 332.1984
718 333.2057 332.1984
719 333.2057 332.1984
720 333.2058 332.1985
721 333.2058 332.1985
722 333.2060 332.1987
723 333.2060 332.1987
724 333.2064 332.1991
725 333.2268 332.2195
726 334.2243 333.2170
727 334.2244 333.2171
728 335.0042 333.9969
729 335.0737 334.0664
730 335.0919 334.0846
731 335.1096 334.1023
732 335.1099 334.1026
733 335.1100 334.1027
734 335.1102 334.1029
735 335.1106 334.1033
736 335.1109 334.1036
737 335.1115 334.1042
738 335.1116 334.1043
739 335.1118 334.1045
740 335.1121 334.1048
741 335.1472 334.1399
742 335.1475 334.1402
743 335.1475 334.1402
744 335.1476 334.1403
745 335.1477 334.1404
746 335.1478 334.1405
747 335.1478 334.1405
748 335.1479 334.1406
749 335.1479 334.1406
750 335.1480 334.1407
751 335.1482 334.1409
752 335.1483 334.1410
753 335.1484 334.1411
754 335.1485 334.1412
755 335.1486 334.1413
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