MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
556 325.1267 324.1194
557 325.1270 324.1197
558 325.1271 324.1198
559 325.1272 324.1199
560 325.1273 324.1200
561 325.1274 324.1201
562 325.1275 324.1202
563 325.1275 324.1202
564 325.1275 324.1202
565 325.1276 324.1203
566 325.1277 324.1204
567 325.1279 324.1206
568 325.1632 324.1559
569 325.1633 324.1560
570 325.1635 324.1562
571 325.1636 324.1563
572 325.1636 324.1563
573 325.1637 324.1564
574 325.1637 324.1564
575 325.1641 324.1568
576 325.1642 324.1569
577 325.1642 324.1569
578 325.1643 324.1570
579 325.1644 324.1571
580 325.1821 324.1748
581 325.1822 324.1749
582 325.1824 324.1751
583 325.1829 324.1756
584 325.2013 324.1940
585 325.2018 324.1945
586 326.1486 325.1413
587 327.0756 326.0683
588 327.0757 326.0684
589 327.0758 326.0685
590 327.0763 326.0690
591 327.0765 326.0692
592 327.0767 326.0694
593 327.1050 326.0977
594 327.1052 326.0979
595 327.1060 326.0987
596 327.1066 326.0993
597 327.1067 326.0994
598 327.1136 326.1063
599 327.1228 326.1155
600 327.1235 326.1162
601 327.1422 326.1349
602 327.1423 326.1350
603 327.1425 326.1352
604 327.1425 326.1352
605 327.1425 326.1352
606 327.1427 326.1354
607 327.1429 326.1356
608 327.1430 326.1357
609 327.1430 326.1357
610 327.1431 326.1358
611 327.1432 326.1359
612 327.1432 326.1359
613 327.1433 326.1360
614 327.1434 326.1361
615 327.1434 326.1361
616 327.1435 326.1362
617 327.1436 326.1363
618 327.1786 326.1713
619 327.1787 326.1714
620 327.1789 326.1716
621 327.1793 326.1720
622 327.1794 326.1721
623 327.1798 326.1725
624 327.1799 326.1726
625 327.1800 326.1727
626 327.2003 326.1930
627 327.2177 326.2104
628 328.1655 327.1582
629 328.2139 327.2066
630 328.2139 327.2066
631 328.6625 327.6552
632 328.9870 327.9797
633 329.0555 328.0482
634 329.0556 328.0483
635 329.0556 328.0483
636 329.0911 328.0838
637 329.0914 328.0841
638 329.0930 328.0857
639 329.1223 328.1150
640 329.1580 328.1507
641 329.1581 328.1508
642 329.1582 328.1509
643 329.1583 328.1510
644 329.1585 328.1512
645 329.1586 328.1513
646 329.1587 328.1514
647 329.1588 328.1515
648 329.1591 328.1518
649 329.1591 328.1518
650 329.1591 328.1518
651 329.1592 328.1519
652 329.1945 328.1872
653 329.1956 328.1883
654 330.0416 329.0343
655 331.0712 330.0639
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