MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
456 321.1328 320.1255
457 321.1328 320.1255
458 321.1328 320.1255
459 321.1329 320.1256
460 321.1680 320.1607
461 321.1682 320.1609
462 321.1684 320.1611
463 321.1684 320.1611
464 321.1685 320.1612
465 321.1687 320.1614
466 321.1687 320.1614
467 321.1688 320.1615
468 321.1688 320.1615
469 321.1690 320.1617
470 321.1692 320.1619
471 321.1693 320.1620
472 321.1693 320.1620
473 321.1693 320.1620
474 321.1694 320.1621
475 321.1694 320.1621
476 321.1694 320.1621
477 321.1694 320.1621
478 321.1695 320.1622
479 321.1696 320.1623
480 321.2059 320.1986
481 321.2060 320.1987
482 321.2060 320.1987
483 321.2062 320.1989
484 321.2423 320.2350
485 321.2424 320.2351
486 321.2426 320.2353
487 322.1562 321.1489
488 322.1565 321.1492
489 322.1565 321.1492
490 323.0186 322.0113
491 323.0485 322.0412
492 323.1105 322.1032
493 323.1105 322.1032
494 323.1107 322.1034
495 323.1108 322.1035
496 323.1111 322.1038
497 323.1111 322.1038
498 323.1115 322.1042
499 323.1115 322.1042
500 323.1115 322.1042
501 323.1115 322.1042
502 323.1116 322.1043
503 323.1118 322.1045
504 323.1472 322.1399
505 323.1472 322.1399
506 323.1474 322.1401
507 323.1475 322.1402
508 323.1475 322.1402
509 323.1476 322.1403
510 323.1476 322.1403
511 323.1477 322.1404
512 323.1479 322.1406
513 323.1479 322.1406
514 323.1479 322.1406
515 323.1480 322.1407
516 323.1482 322.1409
517 323.1483 322.1410
518 323.1484 322.1411
519 323.1486 322.1413
520 323.1486 322.1413
521 323.1486 322.1413
522 323.1487 322.1414
523 323.1487 322.1414
524 323.1490 322.1417
525 323.1657 322.1584
526 323.1662 322.1589
527 323.1664 322.1591
528 323.1666 322.1593
529 323.1839 322.1766
530 323.1841 322.1768
531 323.1841 322.1768
532 323.1843 322.1770
533 323.1844 322.1771
534 323.1845 322.1772
535 323.1845 322.1772
536 323.1846 322.1773
537 323.1850 322.1777
538 323.1851 322.1778
539 323.1852 322.1779
540 323.1852 322.1779
541 323.1852 322.1779
542 323.1853 322.1780
543 323.1853 322.1780
544 323.1854 322.1781
545 323.1855 322.1782
546 323.2048 322.1975
547 325.0612 324.0539
548 325.0905 324.0832
549 325.0988 324.0915
550 325.0999 324.0926
551 325.1068 324.0995
552 325.1072 324.0999
553 325.1265 324.1192
554 325.1266 324.1193
555 325.1266 324.1193
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