MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4271)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2698 817.4434 816.4361 816.4341 2.48 0 19 0.065 +1Score > 20 indicates identity EEIASLR
2631 358.7021 715.3896 715.3864 4.50 0 18 0.079 +1Score > 20 indicates identity LAEDLR
2837 473.7457 945.4768 945.4953 -19.5 0 18 0.085 +1Score > 20 indicates identity EVMEALVR
2697 817.4430 816.4357 816.4341 1.99 0 18 0.085 +1Score > 20 indicates identity NVSNLLR + 2 Deamidated (NQ)
2747 889.4398 888.4325 888.4202 13.9 1 17 0.094 +1Score > 19 indicates identity NHGYNKR + Deamidated (NQ)
2348 475.2275 474.2202 474.2187 3.30 0 17 0.057 +1Score > 17 indicates identity DGAGR
2457 574.2957 573.2884 573.2871 2.37 0 16 0.084 +1Score > 18 indicates identity DAVNR
2431 547.3050 546.2977 546.2948 5.38 0 16 0.18 +1Score > 21 indicates identity AMIGR
2763 904.4530 903.4457 903.4298 17.7 0 16 0.13 +1Score > 19 indicates identity SDAELVDR
2687 788.3921 787.3848 787.3824 3.08 0 16 0.071 +1Score > 17 indicates identity EQNQIR + Deamidated (NQ)
2410 531.2905 530.2832 530.2812 3.75 0 15 0.28 +1Score > 22 indicates identity IGANR + Deamidated (NQ)
3447 345.9420 1379.7389 1379.7483 -6.80 0 15 0.072 +1Score > 16 indicates identity IIFCGTLTAGSLK
3305 437.2300 1308.6682 1308.6673 0.63 0 14 0.13 +1Score > 18 indicates identity LSSLQQSYLNR + Deamidated (NQ)
2835 946.4815 945.4742 945.4880 -14.5 0 14 0.17 +1Score > 19 indicates identity ATVDSIGQR
3306 437.2318 1308.6736 1308.6673 4.76 0 14 0.12 +1Score > 17 indicates identity LSSLQQSYLNR + Deamidated (NQ)
2674 380.2240 758.4334 758.4286 6.37 0 14 0.21 +1Score > 20 indicates identity LAAQLSR + Deamidated (NQ)
3575 484.5695 1450.6867 1450.6987 -8.26 1 14 0.18 +1Score > 19 indicates identity QRMQNATNFLAR + 2 Deamidated (NQ)
2691 807.4048 806.3975 806.4075 -12.4 0 14 0.18 +1Score > 19 indicates identity FAWEVR
3135 595.3235 1188.6324 1188.6251 6.18 0 14 0.18 +1Score > 19 indicates identity ANPNIGVSVYR
3791 886.5048 1770.9950 1770.9767 10.4 1 14 0.065 +1Score > 14 indicates identity EPDLNLLQFLQKLAK + 2 Deamidated (NQ)
3491 349.9413 1395.7361 1395.7358 0.24 1 14 0.12 +1Score > 17 indicates identity THDNKLQVEAIK + Deamidated (NQ)
2645 726.3635 725.3562 725.3530 4.41 1 13 0.09 +1Score > 16 indicates identity KMFER + Oxidation (M)
2675 760.4225 759.4152 759.4173 -2.79 1 13 0.2 +1Score > 20 indicates identity
Score > 19 indicates homology		 RMANLR
3151 401.5377 1201.5913 1201.5985 -6.05 1 13 0.22 +1Score > 19 indicates identity NAALNEMRQR
2412 531.3168 530.3095 530.3064 5.93 0 13 0.53 +1Score > 23 indicates identity AELAK
2381 503.2595 502.2522 502.2500 4.52 0 13 0.23 +1Score > 19 indicates identity SSGPR
2740 879.4602 878.4529 878.4531 -0.23 1 13 0.2 +1Score > 18 indicates identity MGSEISKK
3516 474.2267 1419.6583 1419.6816 -16.5 0 13 0.19 +1Score > 18 indicates identity EMNVQFPAQTVR + Deamidated (NQ)
2836 473.7450 945.4754 945.4590 17.4 0 13 0.24 +1Score > 19 indicates identity IPQQGMVR + 2 Deamidated (NQ); Oxidation (M)
4171 706.6898 2117.0476 2117.0276 9.42 1 13 0.22 +1Score > 19 indicates identity AIISDVNASDEDRWNAVLK + 2 Deamidated (NQ)
2947 505.7473 1009.4800 1009.4651 14.8 0 12 0.12 +1Score > 16 indicates identity GMVFEQQR + Oxidation (M)
2524 324.1750 646.3354 646.3286 10.6 1 12 0.29 +1Score > 19 indicates identity KNEEK
2719 842.4044 841.3971 841.3977 -0.69 1 12 0.15 +1Score > 16 indicates identity DVCHRR
3340 665.3434 1328.6722 1328.6738 -1.14 1 12 0.25 +1Score > 18 indicates identity YQKINAHHYR
3314 657.3588 1312.7030 1312.7099 -5.20 1 12 0.21 +1Score > 18 indicates identity QARLDAAAQLEK
3077 550.2734 1098.5322 1098.5418 -8.66 1 12 0.19 +1Score > 17 indicates identity QAGPDGERLR + Deamidated (NQ)
2902 329.1945 984.5617 984.5604 1.33 1 12 0.24 +1Score > 18 indicates identity TPKEIIER
3489 349.9410 1395.7349 1395.7358 -0.62 1 12 0.19 +1Score > 17 indicates identity THDNKLQVEAIK + Deamidated (NQ)
2634 359.6676 717.3206 717.3293 -12.1 0 12 0.13 +1Score > 15 indicates identity ENDAIR + Deamidated (NQ)
3351 447.2160 1338.6262 1338.6350 -6.61 1 12 0.2 +1Score > 17 indicates identity KDSGFQMNQLR + Oxidation (M)
4094 673.0200 2016.0382 2016.0058 16.0 1 11 0.28 +1Score > 18 indicates identity NEINGVQLVGSGKMVQNGR + Deamidated (NQ); Oxidation (M)
2468 590.2563 589.2490 589.2456 5.83 0 11 0.077 +1Score > 13 indicates identity NGDQR + Deamidated (NQ)
3521 474.9126 1421.7160 1421.6973 13.1 0 11 0.11 +1Score > 20 indicates identity
Score > 14 indicates homology		 QEVAPTSSGLMFR
2601 350.2313 698.4480 698.4439 5.95 0 11 0.078 +1Score > 13 indicates identity IGLLAGR
2975 513.7332 1025.4518 1025.4719 -19.6 0 11 0.14 +1Score > 15 indicates identity FWAGFDQR
2594 694.3755 693.3682 693.3558 17.9 1 11 0.29 +1Score > 18 indicates identity NRFTR + Deamidated (NQ)
3304 654.8531 1307.6916 1307.6833 6.36 0 11 0.24 +1Score > 17 indicates identity LSSLQQSYLNR
4044 994.0114 1986.0082 1986.0170 -4.42 1 11 0.14 +1Score > 20 indicates identity
Score > 15 indicates homology		 KLLDVQSFDSHAAQSLAR + Deamidated (NQ)
2877 487.2451 972.4756 972.4876 -12.3 0 11 0.27 +1Score > 17 indicates identity LAEQNQLR + 2 Deamidated (NQ)
3220 629.3165 1256.6184 1256.6010 13.9 0 11 0.3 +1Score > 18 indicates identity LAEHGATEHHR
3366 337.4409 1345.7345 1345.7606 -19.4 1 10 0.25 +1Score > 17 indicates identity VYPLTVGDKVQK
2595 694.3764 693.3691 693.3666 3.70 1 10 0.32 +1Score > 18 indicates identity MKMLR + Oxidation (M)
2834 473.7435 945.4724 945.4590 14.3 0 10 0.37 +1Score > 19 indicates identity IPQQGMVR + 2 Deamidated (NQ); Oxidation (M)
2680 774.4127 773.4054 773.4032 2.94 0 10 0.45 +1Score > 19 indicates identity IEQTQR
3434 459.5768 1375.7086 1375.7242 -11.3 1 10 0.36 +1Score > 18 indicates identity MSAITESKPTRR
2568 675.3451 674.3378 674.3347 4.59 1 10 0.38 +1Score > 19 indicates identity EDKQR
3631 377.9324 1507.7005 1507.7010 -0.35 0 10 0.35 +1Score > 18 indicates identity SLALMQQTEQMAR + 2 Deamidated (NQ)
3365 337.4407 1345.7337 1345.7606 -20.0 1 10 0.26 +1Score > 17 indicates identity VYPLTVGDKVQK
3021 352.1570 1053.4492 1053.4583 -8.66 0 10 0.096 +1Score > 13 indicates identity LGSMGMAQAR + Deamidated (NQ); 2 Oxidation (M)
3292 434.8776 1301.6110 1301.6285 -13.5 0 10 0.27 +1Score > 17 indicates identity GQSIDDMIPAQK
3492 349.9417 1395.7377 1395.7358 1.38 1 10 0.29 +1Score > 17 indicates identity THDNKLQVEAIK + Deamidated (NQ)
2588 692.3394 691.3321 691.3323 -0.24 0 10 0.39 +1Score > 18 indicates identity MAGLER + Oxidation (M)
2605 351.6833 701.3520 701.3530 -1.40 0 10 0.19 +1Score > 15 indicates identity MPADIR
2879 326.5005 976.4797 976.4865 -7.02 0 10 0.5 +1Score > 19 indicates identity NPNLLDYK + Deamidated (NQ)
3507 470.2592 1407.7558 1407.7722 -11.6 1 10 0.27 +1Score > 17 indicates identity DIDLALDAAHKVK
2414 534.2719 533.2646 533.2558 16.6 0 10 0.54 -1Score > 20 indicates identity SSGGAR
2460 577.2804 576.2731 576.2690 7.24 0 10 0.45 +1Score > 19 indicates identity EAAMR
3221 629.3168 1256.6190 1256.6010 14.4 0 9 0.37 +1Score > 18 indicates identity LAEHGATEHHR
3466 464.9208 1391.7406 1391.7482 -5.51 1 9 0.35 +1Score > 17 indicates identity QLALFKEMQGVK + Deamidated (NQ)
2780 457.7834 913.5522 913.5345 19.4 1 9 0.4 +1Score > 18 indicates identity KQQIQLR + Deamidated (NQ)
2743 442.7440 883.4734 883.4763 -3.21 0 9 0.22 +1Score > 15 indicates identity EALNLPAR + Deamidated (NQ)
3355 671.8715 1341.7284 1341.7153 9.79 0 9 0.66 +1Score > 19 indicates identity LLADPHLGHVNR + Deamidated (NQ)
3282 431.8715 1292.5927 1292.6010 -6.46 0 9 0.4 +1Score > 17 indicates identity STGHAGGIHNWR + Deamidated (NQ)
2759 901.4760 900.4687 900.4665 2.51 1 9 0.47 +1Score > 18 indicates identity KEIPSGNR + Deamidated (NQ)
3500 702.8527 1403.6908 1403.6867 2.95 0 9 0.58 +1Score > 19 indicates identity GISDLAQHYLMR + Deamidated (NQ)
2711 415.2375 828.4604 828.4705 -12.1 0 9 0.54 +1Score > 18 indicates identity TTQILPR + Deamidated (NQ)
2608 703.3390 702.3317 702.3296 2.97 0 9 0.26 +1Score > 15 indicates identity AEAQQR + Deamidated (NQ)
2351 475.2642 474.2569 474.2550 4.01 0 8 0.53 +1Score > 18 indicates identity AAASR
2430 546.2554 545.2481 545.2558 -14.0 0 8 0.21 +1Score > 14 indicates identity AGENR
2739 439.1964 876.3782 876.3622 18.3 0 8 0.2 +1Score > 14 indicates identity MNMFFR + 2 Oxidation (M)
2755 447.7368 893.4590 893.4607 -1.82 0 8 0.66 +1Score > 19 indicates identity FEATATVR
2647 727.3770 726.3697 726.3773 -10.4 0 8 0.18 +1Score > 13 indicates identity SSLHGAR
2754 893.4478 892.4405 892.4403 0.29 1 8 0.5 +1Score > 18 indicates identity AAFEDKGR
3104 572.3235 1142.6324 1142.6295 2.58 0 8 0.45 +1Score > 17 indicates identity VNLIASQIQR + 2 Deamidated (NQ)
3588 731.3749 1460.7352 1460.7623 -18.5 1 8 0.85 +1Score > 20 indicates identity IENKLANEGFVAR + Deamidated (NQ)
4076 670.6828 2009.0266 2009.0079 9.31 1 8 0.71 +1Score > 19 indicates identity RWLESQGVDVANGSNHLK
2744 442.7468 883.4790 883.4763 3.12 0 8 0.27 +1Score > 15 indicates identity EALNLPAR + Deamidated (NQ)
3203 627.3008 1252.5870 1252.5692 14.2 0 8 0.47 +1Score > 17 indicates identity SNHYGMIIMR + 2 Oxidation (M)
2570 338.6754 675.3362 675.3374 -1.66 0 8 0.68 +1Score > 19 indicates identity GMLEAR
2404 529.2793 528.2720 528.2656 12.2 0 8 0.16 +1Score > 13 indicates identity PANAR + Deamidated (NQ)
3517 710.8474 1419.6802 1419.6630 12.2 0 8 0.67 +1Score > 19 indicates identity SLTWNGITQQNR + 3 Deamidated (NQ)
2564 672.4080 671.4007 671.3966 6.13 0 8 0.83 +1Score > 20 indicates identity GNVLIR + Deamidated (NQ)
2954 338.1614 1011.4624 1011.4444 17.8 0 8 0.29 +1Score > 15 indicates identity MTPSNYQR + Oxidation (M)
3616 372.6673 1486.6401 1486.6609 -14.0 0 8 0.3 +1Score > 15 indicates identity GMLNELDEEPSPR + Deamidated (NQ)
4245 610.8032 2439.1837 2439.2104 -11.0 0 8 0.92 +1Score > 20 indicates identity FVQPGTQVYDLGCSLGAATLSVR + Deamidated (NQ)
3448 345.9420 1379.7389 1379.7483 -6.80 0 8 0.37 +1Score > 16 indicates identity IIFCGTLTAGSLK
2723 428.7505 855.4864 855.4715 17.5 0 8 0.3 +1Score > 15 indicates identity RPLWQR + Deamidated (NQ)
3034 533.7429 1065.4712 1065.4727 -1.34 0 8 0.3 +1Score > 15 indicates identity GQGAQLNGYR + 3 Deamidated (NQ)
2678 386.7214 771.4282 771.4391 -14.1 0 8 0.68 +1Score > 18 indicates identity LAIYHR
4244 488.8431 2439.1791 2439.2250 -18.8 1 8 0.91 +1Score > 20 indicates identity SNIGHPQGAAGVAGVIKMVMALQR + 3 Deamidated (NQ); 2 Oxidation (M)
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