MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1249__1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:44:49 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,592 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9EX54_STRCO | 4154 | Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 196 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 | |
| 2 | K22E_HUMAN | 114 | Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 188 | Trypsin OS=Sus scrofa PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 168 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 145 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 | |
| 2 | K1C9_HUMAN | 101 | Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS1_ECOLI | 97 | 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | RS1_ECOLI |
97 | 61235 | 9 (5) | 4 (3) | 0.23 | |
| 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1 | |||||||
9 peptide matches (6 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2548 |
1 |
665.3539 | 664.3466 | 664.3432 | 5.20 | 0 | 22 | 0.013 | 7Score > 16 indicates identity |
U | K.ELEFK.V |
| 2549 |
2 |
333.1808 | 664.3470 | 664.3432 | 5.84 | 0 | 12 | 0.15 | 3Score > 16 indicates identity |
U | K.ELEFK.V |
| 3650 |
775.4016 | 1548.7886 | 1548.7784 | 6.63 | 0 | 44 | 0.00016 | 1Score > 19 indicates identity |
U | K.GATVELADGVEGYLR.A | |
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__1.rawScore > 19 indicates identity |
|||||||||||
| 4174 |
710.7042 | 2129.0908 | 2129.0562 | 16.2 | 0 | 36 | 0.001 | 1Score > 19 indicates identity |
U | K.VVNVGDVVEVMVLDIDEER.R + Deamidated (NQ) | |
| 10.4 | 1 | 20 | 0.045 | 2 | VEANEMPGNKIGLLNVHHR + 2 Deamidated (NQ) | ||||||
| 10.4 | 1 | 20 | 0.045 | 2 | VEANEMPGNKIGLLNVHHR + 2 Deamidated (NQ) | ||||||
| 8.43 | 1 | 4 | 1.7 | 4 | AVKVAFVFSGQGTQYPGMAR + Oxidation (M) | ||||||
| -2.65 | 1 | 1 | 3.2 | 5 | KTAGVLGDDLSLNAQQVSGVR + 2 Deamidated (NQ) | ||||||
| -2.65 | 1 | 1 | 3.2 | 5 | KTAGVLGDDLSLNAQQVSGVR + 2 Deamidated (NQ) | ||||||
| 4175 |
1065.5533 | 2129.0920 | 2129.0562 | 16.8 | 0 | 46 | 9.7e-005 | 1Score > 19 indicates identity |
U | K.VVNVGDVVEVMVLDIDEER.R + Deamidated (NQ) | |
| 4468 |
1191.5579 | 3571.6519 | 3571.6011 | 14.2 | 0 | 8 | 0.49 | 1Score > 18 indicates identity |
U | R.VTNLTDYGCFVEIEEGVEGLVHVSEMDWTNK.N + 2 Deamidated (NQ) | |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_ECOLI | 65 | 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPA_ECOLI | 58 | Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPB_ECOLI | 44 | Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_ECOLI | 42 | 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2 |
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