MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 Q9EX54_STRCO 4154 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 K2C1_HUMAN 196 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
2 K22E_HUMAN 114 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 188 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 EFTU1_ECOLI 168 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

-5

Accession Score Description
Family member distances as a dendrogram 1 K1C10_HUMAN 145 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
2 K1C9_HUMAN 101 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3
Cut threshold

Score Mass Matches Sequences emPAI
K1C10_HUMAN 145 59020 16 (9) 15 (9) 0.63
Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
K1C9_HUMAN 101 62255 12 (7) 6 (6) 0.36
Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3

-27 peptide matches (24 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
2662   373.2154 744.4162 744.4130 4.37 0 24 0.029 +1Score > 21 indicates identity U X K.EVTQLR.H
2692   405.2272 808.4398 808.4330 8.42 0 20 0.022 +1Score > 16 indicates identity U X X R.LASYLDK.V
2775   457.2093 912.4040 912.4011 3.21 0 44 4.8e-005 +1Score > 13 indicates identity U X R.MTLDDFR.I + Oxidation (M)
2928   498.2669 994.5192 994.5123 6.94 1 4 1 +1Score > 17 indicates identity U X K.IKEWYEK.H
2938   335.1900 1002.5482 1002.5458 2.39 1 22 0.022 +1Score > 18 indicates identity U X K.SEITELRR.N
2939   502.2814 1002.5482 1002.5458 2.47 1 2 2.4 +2Score > 18 indicates identity U X K.SEITELRR.N
3026   530.7888 1059.5630 1059.5560 6.62 0 42 0.00028 +1Score > 19 indicates identity U X K.TLLDIDNTR.M
3032   355.5424 1063.6054 1063.6026 2.63 1 10 0.14 +1Score > 14 indicates identity U X R.LASYLDKVR.A
3083   553.7695 1105.5244 1105.5186 5.29 0 39 0.00049 +1Score > 19 indicates identity U X K.VTMQNLNDR.L + Oxidation (M)
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__1.raw

Score > 19 indicates identity
3117   583.3011 1164.5876 1164.5775 8.74 0 14 0.18 -1Score > 19 indicates identity U X R.LENEIQTYR.S
No other peptide matches in query
3144   401.2089 1200.6049 1200.6098 -4.14 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology		 U X R.QSVEADINGLR.R
3175   616.8060 1231.5974 1231.5906 5.59 0 37 0.00091 +1Score > 19 indicates identity U X R.SGGGGGGGLGSGGSIR.S
3176   412.2335 1233.6787 1233.6717 5.66 1 18 0.036 +1Score > 16 indicates identity U X R.LKYENEVALR.Q
3300 +1 436.5662 1306.6768 1306.6703 4.94 1 21 0.026 +1Score > 18 indicates identity U X R.IKFEMEQNLR.Q
3302   327.6774 1306.6805 1306.6703 7.79 1 1 2.9 +1Score > 18 indicates identity U X R.IKFEMEQNLR.Q
3319   439.5370 1315.5892 1315.5826 4.98 1 3 0.54 +1Score > 13 indicates identity U X K.NHEEEMKDLR.N + Oxidation (M)
3329 +1 331.6708 1322.6541 1322.6652 -8.42 1 1 3.4 +3Score > 18 indicates identity U X R.IKFEMEQNLR.Q + Oxidation (M)
3332 +1 441.8926 1322.6560 1322.6652 -7.00 1 15 0.12 +1Score > 18 indicates identity U X R.IKFEMEQNLR.Q + Oxidation (M)
3380   453.2466 1356.7180 1356.7110 5.17 1 34 0.0019 +1Score > 19 indicates identity U X R.QSVEADINGLRR.V
3453   691.8312 1381.6478 1381.6248 16.6 0 53 1.4e-005 +1Score > 17 indicates identity U X R.ALEESNYELEGK.I + Deamidated (NQ)
3561   478.9302 1433.7688 1433.7626 4.28 1 20 0.034 +1Score > 17 indicates identity U X K.IRLENEIQTYR.S
3622   498.5853 1492.7341 1492.7270 4.76 1 44 0.00017 +1Score > 19 indicates identity U X R.SQYEQLAEQNRK.D
4183   747.7210 2240.1412 2240.1133 12.4 1 37 0.00084 +1Score > 19 indicates identity U X K.ADLEMQIESLTEELAYLKK.N + Deamidated (NQ); Oxidation (M)
4421   969.1377 2904.3913 2904.3593 11.0 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 U X R.NVSTGDVNVEMNAAPGVDLTQLLNNMR.S + Deamidated (NQ); 2 Oxidation (M)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
K1C15_SHEEP 34 48740 5.1
Keratin, type I cytoskeletal 15 OS=Ovis aries GN=KRT15 PE=2 SV=1

+6

Accession Score Description
1 RS1_ECOLI 97 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1

+7

Accession Score Description
1 RL4_ECOLI 65 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

+8

Accession Score Description
1 ISPA_ECOLI 58 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1

+9

Accession Score Description
1 GLPB_ECOLI 44 Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1

+10

Accession Score Description
1 RL7_ECOLI 42 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2
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