MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 Q9EX54_STRCO 4154 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1

-2

Accession Score Description
Family member distances as a dendrogram 1 K2C1_HUMAN 196 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
2 K22E_HUMAN 114 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2
Cut threshold

Score Mass Matches Sequences emPAI
K2C1_HUMAN 196 66170 12 (7) 12 (7) 0.40
Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
K22E_HUMAN 114 65678 8 (6) 8 (6) 0.34
Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2

-18 peptide matches (18 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
2567   337.1982 672.3818 672.3806 1.81 0 10 0.14 +1Score > 20 indicates identity
Score > 14 indicates homology		 U X K.VDLQAK.L
2609   352.2047 702.3948 702.3912 5.21 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology		 U X K.VELQSK.V
2612   352.6962 703.3778 703.3752 3.77 0 8 0.76 +1Score > 19 indicates identity U X R.LDSELK.N
2672   379.7318 757.4490 757.4446 5.84 1 9 0.36 +1Score > 18 indicates identity U X R.RVDQLK.S
2737   437.7558 873.4970 873.4920 5.81 0 28 0.0084 +1Score > 19 indicates identity U X R.SLVNLGGSK.S
2878   487.2729 972.5312 972.5240 7.48 0 39 0.00066 +1Score > 20 indicates identity U X X K.IEISELNR.V
2985   517.2650 1032.5154 1032.5087 6.51 0 17 0.11 +1Score > 19 indicates identity U X R.TLLEGEESR.M
2992   519.2698 1036.5250 1036.5189 5.93 0 26 0.011 +1Score > 19 indicates identity U X R.YLDGLTAER.T
3000   521.2858 1040.5570 1040.5502 6.56 0 17 0.046 +1Score > 16 indicates identity U X K.VDPEIQNVK.A
3033   533.2640 1064.5134 1064.5138 -0.34 0 41 0.00028 +1Score > 18 indicates identity U X K.AQYEDIAQK.S
3085   554.2753 1106.5360 1106.5356 0.41 0 27 0.0077 +1Score > 18 indicates identity U X K.AQYEEIAQR.S
3122   590.3074 1178.6002 1178.5931 6.04 0 62 2.6e-006 +1Score > 19 indicates identity U X K.YEELQITAGR.H
3293   651.8648 1301.7150 1301.7078 5.55 0 46 8e-005 +1Score > 18 indicates identity U X R.SLDLDSIIAEVK.A
3341   665.3716 1328.7286 1328.7187 7.47 0 19 0.057 +1Score > 19 indicates identity U X R.NLDLDSIIAEVK.A
3454   692.3498 1382.6850 1382.6830 1.47 0 48 5.4e-005 +1Score > 18 indicates identity U X K.SLNNQFASFIDK.V
3468   465.2505 1392.7297 1392.7249 3.45 1 14 0.11 +1Score > 17 indicates identity U X R.TNAENEFVTIKK.D
3587   730.9089 1459.8032 1459.7922 7.55 0 52 1.5e-005 +1Score > 17 indicates identity U X K.VDLLNQEIEFLK.V
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__1.raw

Score > 19 indicates identity
3604   738.4011 1474.7876 1474.7780 6.55 0 43 0.0002 -1Score > 19 indicates identity U X X R.FLEQQNQVLQTK.W
No other peptide matches in query

+3

Accession Score Description
1 TRYP_PIG 188 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 EFTU1_ECOLI 168 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 K1C10_HUMAN 145 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
2 K1C9_HUMAN 101 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3

+6

Accession Score Description
1 RS1_ECOLI 97 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1

+7

Accession Score Description
1 RL4_ECOLI 65 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

+8

Accession Score Description
1 ISPA_ECOLI 58 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1

+9

Accession Score Description
1 GLPB_ECOLI 44 Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1

+10

Accession Score Description
1 RL7_ECOLI 42 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2
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