MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:44:49 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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-1

Accession Score Description
1 Q9EX54_STRCO 4154 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1
Score Mass Matches Sequences emPAI
1.1 Q9EX54_STRCO 4154 46170 204 (156) 38 (33) 61.93
Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1

-204 peptide matches (73 non-duplicate, 131 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2424 +1 541.3469 540.3396 540.3384 2.30 0 16 0.026 +1Score > 13 indicates identity U R.GVPLR.V
2513 +4 321.7195 641.4244 641.4224 3.16 0 33 0.00068 +1Score > 14 indicates identity U R.IIGIAR.D
2516 +1 642.4322 641.4249 641.4224 3.90 0 29 0.0015 +1Score > 14 indicates identity U R.IIGIAR.D
2534 +1 330.6708 659.3270 659.3238 4.89 0 30 0.0054 +1Score > 19 indicates identity U R.ENLER.Y
2535   660.3441 659.3368 659.3238 19.7 0 9 0.64 +1Score > 21 indicates identity
Score > 20 indicates homology		 U R.ENLER.Y
2537   660.3497 659.3424 659.3391 5.05 0 16 0.1 +2Score > 19 indicates identity U K.WVAER.I
2538 +2 330.6785 659.3424 659.3391 5.08 0 37 0.00098 +1Score > 19 indicates identity U K.WVAER.I
2546 +2 333.1802 664.3458 664.3432 4.03 0 22 0.016 +1Score > 16 indicates identity U R.ELFEK.Y
2548 +1 665.3539 664.3466 664.3432 5.20 0 37 0.0005 +1Score > 16 indicates identity U R.ELFEK.Y
2562   336.6891 671.3636 671.3602 5.09 0 12 0.15 +1Score > 16 indicates identity U R.TQGPAAK.T
2750 +1 890.5131 889.5058 889.5022 4.13 0 25 0.0076 +1Score > 17 indicates identity U R.GLPVSVYR.V
2751 +2 445.7602 889.5058 889.5022 4.16 0 39 0.00035 +1Score > 17 indicates identity U R.GLPVSVYR.V
2799 +3 310.5105 928.5097 928.5090 0.74 1 33 0.0022 +1Score > 18 indicates identity U R.LRENLER.Y
2801 +2 465.2629 928.5112 928.5090 2.44 1 33 0.0018 +1Score > 18 indicates identity U R.LRENLER.Y
2853 +3 319.1770 954.5092 954.5069 2.35 0 40 0.00024 +1Score > 17 indicates identity U R.ATVHCLVR.G
2855   955.5176 954.5103 954.5069 3.56 0 13 0.13 +1Score > 17 indicates identity U R.ATVHCLVR.G
2857 +1 478.2633 954.5120 954.5069 5.37 0 54 9.8e-006 +1Score > 17 indicates identity U R.ATVHCLVR.G
2907 +1 988.5101 987.5028 987.4985 4.34 0 25 0.014 +1Score > 19 indicates identity U R.VDVISGDQR.N
2911 +4 494.7593 987.5040 987.4985 5.58 0 61 5e-006 +1Score > 20 indicates identity U R.VDVISGDQR.N
2914 +4 496.7745 991.5344 991.5338 0.62 0 51 2.1e-005 +1Score > 17 indicates identity U R.LPSGYLQSK.W
2917 +1 992.5453 991.5380 991.5338 4.23 0 48 5.3e-005 +1Score > 17 indicates identity U R.LPSGYLQSK.W
2948   338.1529 1011.4369 1011.4370 -0.093 0 5 0.33 +1Score > 13 indicates identity U R.GEDEAGAHAR.L
2949   506.7281 1011.4416 1011.4370 4.63 0 4 0.39 +1Score > 13 indicates identity U R.GEDEAGAHAR.L
3002 +3 521.7632 1041.5118 1041.5091 2.64 0 54 6.5e-006 +1Score > 15 indicates identity U R.VTDPTGPAER.L
3003   1042.5194 1041.5121 1041.5091 2.91 0 20 0.019 +1Score > 15 indicates identity U R.VTDPTGPAER.L
3057   361.8676 1082.5810 1082.5760 4.58 1 12 0.12 +1Score > 15 indicates identity U R.ELFEKYVR.F
3112 +2 581.3248 1160.6350 1160.6302 4.17 1 49 5.1e-005 +1Score > 18 indicates identity U R.DRGLPVSVYR.V
3116 +2 387.8867 1160.6383 1160.6302 6.95 1 38 0.00073 +1Score > 19 indicates identity U R.DRGLPVSVYR.V
3146 +1 401.5355 1201.5847 1201.5840 0.57 0 8 0.76 +1Score > 19 indicates identity U R.TVDAVYHNGAR.V
3148   601.8011 1201.5876 1201.5840 3.05 0 22 0.031 +1Score > 19 indicates identity U R.TVDAVYHNGAR.V
3160 +4 610.3018 1218.5890 1218.5881 0.80 0 78 6.8e-008 +1Score > 19 indicates identity U K.TDVPDFAAEVR.L
3205 +2 627.3657 1252.7168 1252.7139 2.32 0 89 1.6e-009 +1Score > 13 indicates identity U R.LSVVVGDLAQPR.L
3206   418.5798 1252.7176 1252.7139 2.90 0 53 6.4e-006 +1Score > 13 indicates identity U R.LSVVVGDLAQPR.L
3248 +5 640.3113 1278.6080 1278.6033 3.68 0 28 0.0063 +1Score > 18 indicates identity U R.FFVETGHFPAAG.-
3271 +4 643.3593 1284.7040 1284.7037 0.24 0 65 1.3e-006 +1Score > 19 indicates identity U K.AANVLGTEEILR.L
3276 +1 429.2437 1284.7093 1284.7037 4.31 0 65 1e-006 +1Score > 18 indicates identity U K.AANVLGTEEILR.L
3336 +1 662.7949 1323.5752 1323.5732 1.58 0 89 2.1e-009 +1Score > 15 indicates identity U R.YGVWDEVDADR.L
3397 +8 341.9431 1363.7433 1363.7401 2.36 0 20 0.013 +1Score > 14 indicates identity U R.VHWLHPYATLK.A
3399 +9 455.5886 1363.7440 1363.7401 2.85 0 41 0.0001 +1Score > 14 indicates identity U R.VHWLHPYATLK.A
3402 +1 682.8795 1363.7444 1363.7401 3.20 0 36 0.00032 +1Score > 14 indicates identity U R.VHWLHPYATLK.A
3422 +2 683.8759 1365.7372 1365.7365 0.56 0 85 6.1e-009 +1Score > 16 indicates identity U K.GLLQAGGVPADVGGR.F
3426 +1 456.2560 1365.7462 1365.7365 7.10 0 47 3.6e-005 +1Score > 15 indicates identity U K.GLLQAGGVPADVGGR.F
3535 +3 714.3688 1426.7230 1426.7205 1.81 0 101 3.4e-010 +1Score > 19 indicates identity U R.LGLAEDAFDHLAR.T
3536 +16 476.5818 1426.7236 1426.7205 2.18 0 74 1.7e-007 +1Score > 19 indicates identity U R.LGLAEDAFDHLAR.T
3654 +1 522.2899 1563.8479 1563.8369 7.01 1 43 0.00015 +1Score > 17 indicates identity U R.GVPLRVTDPTGPAER.L
3662   545.2958 1632.8656 1632.8624 1.96 1 57 7e-006 +1Score > 18 indicates identity U R.LPSGYLQSKWVAER.I
3689 +3 571.9950 1712.9632 1712.9614 1.05 0 66 2.9e-007 +1Score > 13 indicates identity U R.FHLLPVDYVSAAILR.I
3690   429.2485 1712.9649 1712.9614 2.06 0 71 9.5e-008 +1Score > 13 indicates identity U R.FHLLPVDYVSAAILR.I
3691 +5 857.4899 1712.9652 1712.9614 2.27 0 68 1.7e-007 +1Score > 13 indicates identity U R.FHLLPVDYVSAAILR.I
3709   863.4197 1724.8248 1724.8192 3.27 0 73 1.8e-007 +1Score > 18 indicates identity U R.NGACQTSDFVWLSIK.G
3712 +1 863.9172 1725.8198 1725.8032 9.63 0 87 5.4e-009 +1Score > 17 indicates identity U R.NGACQTSDFVWLSIK.G + Deamidated (NQ)
3713 +1 576.2852 1725.8338 1725.8032 17.7 0 39 0.00034 +1Score > 17 indicates identity U R.NGACQTSDFVWLSIK.G + Deamidated (NQ)
3714   576.6140 1726.8202 1726.7872 19.1 0 24 0.01 +1Score > 17 indicates identity U R.NGACQTSDFVWLSIK.G + 2 Deamidated (NQ)
3807 +1 597.3342 1788.9808 1788.9709 5.52 0 90 1.4e-009 +1Score > 14 indicates identity U R.HVLLTGASGFLGAFLMR.D
3823   903.4950 1804.9754 1804.9658 5.33 0 44 8.8e-005 +1Score > 16 indicates identity U R.HVLLTGASGFLGAFLMR.D + Oxidation (M)
3824 +2 602.6667 1804.9783 1804.9658 6.90 0 59 3e-006 +1Score > 16 indicates identity U R.HVLLTGASGFLGAFLMR.D + Oxidation (M)
3915   624.9906 1871.9500 1871.9377 6.53 1 41 0.00032 +1Score > 19 indicates identity U R.TQGPAAKTDVPDFAAEVR.L
3919   938.4439 1874.8732 1874.8475 13.7 0 79 4e-008 +1Score > 18 indicates identity U R.SFGYSLTELDWNTWR.A + Deamidated (NQ)
3920 +1 625.9651 1874.8735 1874.8475 13.8 0 45 0.00012 +1Score > 18 indicates identity U R.SFGYSLTELDWNTWR.A + Deamidated (NQ)
3989   650.3217 1947.9433 1947.9333 5.10 1 2 3.1 +1Score > 19 indicates identity U R.ATVHCLVRGEDEAGAHAR.L
3991 +1 390.5964 1947.9456 1947.9333 6.31 1 26 0.013 +1Score > 19 indicates identity U R.ATVHCLVRGEDEAGAHAR.L
3992   487.9942 1947.9477 1947.9333 7.37 1 35 0.0015 +1Score > 19 indicates identity U R.ATVHCLVRGEDEAGAHAR.L
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__1.raw

Score > 19 indicates identity
4072   670.3503 2008.0291 2008.0225 3.26 0 42 0.00023 -1Score > 19 indicates identity U R.LAEDIRPAADVVSVADDPR.H
No other peptide matches in query
4073 +1 1005.0219 2008.0292 2008.0225 3.35 0 40 0.0004 +1Score > 19 indicates identity U R.LAEDIRPAADVVSVADDPR.H
4074   503.0151 2008.0313 2008.0225 4.37 0 9 0.5 +1Score > 19 indicates identity U R.LAEDIRPAADVVSVADDPR.H
4092   672.6874 2015.0404 2015.0324 3.97 1 25 0.011 +1Score > 18 indicates identity U R.VTDPTGPAERLPSGYLQSK.W
4095   673.0226 2016.0460 2016.0164 14.7 1 1 2.8 +3Score > 18 indicates identity U R.VTDPTGPAERLPSGYLQSK.W + Deamidated (NQ)
4207 +2 796.4291 2386.2655 2386.2393 11.0 0 37 0.00067 +1Score > 17 indicates identity U R.IASRPSAAGGTFHLFNPSSISLR.E + Deamidated (NQ)
4208 +5 597.5739 2386.2665 2386.2393 11.4 0 55 1.1e-005 +1Score > 17 indicates identity U R.IASRPSAAGGTFHLFNPSSISLR.E + Deamidated (NQ)
4303   640.5796 2558.2893 2558.2765 4.99 1 27 0.01 +1Score > 20 indicates identity U R.YGVWDEVDADRLSVVVGDLAQPR.L
4306 +8 854.1047 2559.2923 2559.2605 12.4 1 35 0.0016 +1Score > 19 indicates identity U R.YGVWDEVDADRLSVVVGDLAQPR.L + Deamidated (NQ)
4394   900.1105 2697.3097 2697.2592 18.7 1 16 0.097 +1Score > 19 indicates identity U R.VDVISGDQRNGACQTSDFVWLSIK.G + 3 Deamidated (NQ)
4432   737.1339 2944.5065 2944.4977 2.98 1 7 0.87 +1Score > 19 indicates identity U R.IASRPSAAGGTFHLFNPSSISLRECIR.H + Deamidated (NQ)

2 subsets and intersections (3 subset proteins in total)

Score Mass Subset of
YAHA_ECOLI 71 0 1.1
description
A4U8R3_9BACT 16 362145 1.1
SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1
1 sameset of A4U8R3_9BACT
Q70HZ8_9ACTN 16 0
description

+2

Accession Score Description
Family member distances as a dendrogram 1 K2C1_HUMAN 196 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
2 K22E_HUMAN 114 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 188 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 EFTU1_ECOLI 168 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 K1C10_HUMAN 145 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
2 K1C9_HUMAN 101 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3

+6

Accession Score Description
1 RS1_ECOLI 97 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1

+7

Accession Score Description
1 RL4_ECOLI 65 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

+8

Accession Score Description
1 ISPA_ECOLI 58 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1

+9

Accession Score Description
1 GLPB_ECOLI 44 Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1

+10

Accession Score Description
1 RL7_ECOLI 42 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2
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