MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1249__1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues) |
| Timestamp | : | 23 Jun 2025 at 17:44:49 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,592 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9EX54_STRCO | 4154 | Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 196 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 | |
| 2 | K22E_HUMAN | 114 | Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 188 | Trypsin OS=Sus scrofa PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 168 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | EFTU1_ECOLI |
168 | 43427 | 9 (6) | 2 (1) | 0.16 | |
| Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 | |||||||
| 1 sameset of EFTU1_ECOLI | |||||||
| EFTU2_ECOLI |
168 | 43457 | 9 (6) | 2 (1) | 0.16 | ||
| Elongation factor Tu 2 OS=Escherichia coli (strain K12) GN=tufB PE=1 SV=1 | |||||||
9 peptide matches (3 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3801 |
445.9872 | 1779.9197 | 1779.9375 | -10.0 | 0 | 6 | 0.73 | 1Score > 18 indicates identity |
U | K.MVVTLIHPIAMDDGLR.F | |
| 4401 |
5 |
910.7898 | 2729.3476 | 2729.3186 | 10.6 | 0 | 76 | 1.2e-007 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) |
| 4402 |
910.7903 | 2729.3491 | 2729.3186 | 11.1 | 0 | (45) | 0.00015 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | |
| 4403 |
910.7904 | 2729.3494 | 2729.3186 | 11.3 | 0 | (20) | 0.049 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | |
| 4404 |
910.7906 | 2729.3500 | 2729.3186 | 11.5 | 0 | (44) | 0.00021 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | |
| 4405 |
910.7918 | 2729.3536 | 2729.3186 | 12.8 | 0 | (7) | 0.87 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | |
| 4406 |
910.7929 | 2729.3569 | 2729.3186 | 14.0 | 0 | (6) | 1.1 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | |
| 4407 |
1 |
916.1223 | 2745.3451 | 2745.3136 | 11.5 | 0 | 45 | 0.00014 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ); Oxidation (M) |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 145 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 | |
| 2 | K1C9_HUMAN | 101 | Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS1_ECOLI | 97 | 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_ECOLI | 65 | 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPA_ECOLI | 58 | Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPB_ECOLI | 44 | Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_ECOLI | 42 | 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2 |
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