MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
324 317.1745 316.1672
325 317.1944 316.1871
326 317.1948 316.1875
327 318.1196 317.1123
328 318.1691 317.1618
329 318.1917 317.1844
330 318.1918 317.1845
331 318.1920 317.1847
332 318.1928 317.1855
333 318.8782 317.8709
334 319.0394 318.0321
335 319.0492 318.0419
336 319.1155 318.1082
337 319.1157 318.1084
338 319.1157 318.1084
339 319.1157 318.1084
340 319.1158 318.1085
341 319.1159 318.1086
342 319.1162 318.1089
343 319.1164 318.1091
344 319.1165 318.1092
345 319.1169 318.1096
346 319.1522 318.1449
347 319.1523 318.1450
348 319.1523 318.1450
349 319.1524 318.1451
350 319.1524 318.1451
351 319.1527 318.1454
352 319.1528 318.1455
353 319.1528 318.1455
354 319.1530 318.1457
355 319.1531 318.1458
356 319.1532 318.1459
357 319.1533 318.1460
358 319.1535 318.1462
359 319.1536 318.1463
360 319.1536 318.1463
361 319.1538 318.1465
362 319.1893 318.1820
363 319.1897 318.1824
364 319.1898 318.1825
365 319.1898 318.1825
366 319.1900 318.1827
367 319.1901 318.1828
368 319.2262 318.2189
369 319.2263 318.2190
370 319.2266 318.2193
371 320.0117 319.0044
372 321.0661 320.0588
373 321.0949 320.0876
374 321.0949 320.0876
375 321.0952 320.0879
376 321.0952 320.0879
377 321.0958 320.0885
378 321.1303 320.1230
379 321.1313 320.1240
380 321.1314 320.1241
381 321.1314 320.1241
382 321.1315 320.1242
383 321.1317 320.1244
384 321.1318 320.1245
385 321.1318 320.1245
386 321.1318 320.1245
387 321.1319 320.1246
388 321.1320 320.1247
389 321.1320 320.1247
390 321.1321 320.1248
391 321.1321 320.1248
392 321.1323 320.1250
393 321.1323 320.1250
394 321.1326 320.1253
395 321.1567 320.1494
396 321.1678 320.1605
397 321.1680 320.1607
398 321.1681 320.1608
399 321.1682 320.1609
400 321.1682 320.1609
401 321.1684 320.1611
402 321.1685 320.1612
403 321.1686 320.1613
404 321.1686 320.1613
405 321.1687 320.1614
406 321.1688 320.1615
407 321.1689 320.1616
408 321.1689 320.1616
409 321.1689 320.1616
410 321.1690 320.1617
411 321.1690 320.1617
412 321.1692 320.1619
413 321.1693 320.1620
414 321.1693 320.1620
415 321.1693 320.1620
416 321.2056 320.1983
417 321.2056 320.1983
418 321.2057 320.1984
419 322.1562 321.1489
420 323.0264 322.0191
421 323.1101 322.1028
422 323.1104 322.1031
423 323.1104 322.1031
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