MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
224 310.0147 309.0074
225 311.0443 310.0370
226 311.0804 310.0731
227 311.0807 310.0734
228 311.0811 310.0738
229 311.0812 310.0739
230 311.0813 310.0740
231 311.0817 310.0744
232 311.0823 310.0750
233 311.0827 310.0754
234 311.0895 310.0822
235 311.0908 310.0835
236 311.1101 310.1028
237 311.1109 310.1036
238 311.1110 310.1037
239 311.1111 310.1038
240 311.1111 310.1038
241 311.1112 310.1039
242 311.1112 310.1039
243 311.1114 310.1041
244 311.1115 310.1042
245 311.1118 310.1045
246 311.1470 310.1397
247 311.1474 310.1401
248 311.1476 310.1403
249 311.1477 310.1404
250 311.1477 310.1404
251 311.1478 310.1405
252 311.1478 310.1405
253 311.1478 310.1405
254 311.1479 310.1406
255 311.1480 310.1407
256 311.1480 310.1407
257 311.1481 310.1408
258 311.1481 310.1408
259 311.1660 310.1587
260 311.1662 310.1589
261 311.1663 310.1590
262 311.1833 310.1760
263 311.1840 310.1767
264 311.2230 310.2157
265 312.0542 311.0469
266 312.1321 311.1248
267 312.1321 311.1248
268 313.0595 312.0522
269 313.0600 312.0527
270 313.0602 312.0529
271 313.0605 312.0532
272 313.0606 312.0533
273 313.0893 312.0820
274 313.0965 312.0892
275 313.0971 312.0898
276 313.0984 312.0911
277 313.1270 312.1197
278 313.1271 312.1198
279 313.1272 312.1199
280 313.1274 312.1201
281 313.1286 312.1213
282 313.1627 312.1554
283 313.1627 312.1554
284 313.1628 312.1555
285 313.1638 312.1565
286 313.1643 312.1570
287 313.1647 312.1574
288 314.0120 313.0047
289 314.0121 313.0048
290 314.1971 313.1898
291 314.1974 313.1901
292 314.1976 313.1903
293 315.0543 314.0470
294 315.0752 314.0679
295 315.0755 314.0682
296 315.0756 314.0683
297 315.1133 314.1060
298 315.1141 314.1068
299 315.1423 314.1350
300 315.1426 314.1353
301 315.1426 314.1353
302 315.1428 314.1355
303 315.1604 314.1531
304 315.1784 314.1711
305 316.0078 315.0005
306 316.1986 315.1913
307 316.2124 315.2051
308 316.2129 315.2056
309 317.0542 316.0469
310 317.0991 316.0918
311 317.0995 316.0922
312 317.1001 316.0928
313 317.1004 316.0931
314 317.1005 316.0932
315 317.1013 316.0940
316 317.1284 316.1211
317 317.1367 316.1294
318 317.1375 316.1302
319 317.1377 316.1304
320 317.1740 316.1667
321 317.1741 316.1668
322 317.1742 316.1669
323 317.1743 316.1670
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