MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4467 798.2430 3188.9429
4468 643.1883 3210.9051
4469 539.8317 3232.9465
4470 647.8004 3233.9656
4472 651.9955 3254.9411
4473 656.4030 3276.9786
4474 547.3361 3277.9729
4475 663.9460 3314.6936
4476 554.5076 3321.0019
4477 665.2081 3321.0041
4478 1111.5510 3331.6312
4479 558.3411 3344.0029
4480 561.8448 3365.0251
4481 674.0140 3365.0336
4482 1124.6459 3370.9159
4483 309.0950 3388.9650
4484 682.8182 3409.0546
4485 569.3514 3410.0647
4486 1139.3213 3414.9421
4487 856.6869 3422.7185
4488 576.5207 3453.0805
4489 691.6243 3453.0851
4490 580.1854 3475.0687
4491 584.0253 3498.1081
4492 700.6304 3498.1156
4493 701.3964 3501.9456
4494 588.8612 3527.1235
4495 709.2334 3541.1306
4496 591.3621 3542.1289
4497 893.5093 3570.0081
4499 598.5333 3585.1561
4500 718.2393 3586.1601
4501 903.0272 3608.0797
4502 602.3648 3608.1451
4503 605.8714 3629.1847
4504 609.5392 3651.1915
4505 613.2086 3673.2079
4507 620.5464 3717.2347
4508 532.0409 3717.2354
4509 934.7023 3734.7801
4510 538.3304 3761.2619
4511 627.8880 3761.2843
4512 628.0521 3762.2689
4513 943.2481 3768.9633
4514 760.4077 3797.0021
4515 544.6205 3805.2926
4516 551.0526 3850.3173
4517 642.7275 3850.3213
4518 551.3384 3852.3179
4519 649.9030 3893.3743
4520 557.3439 3894.3564
4521 650.0692 3894.3715
4522 984.2603 3933.0121
4523 563.4897 3937.3770
4525 569.9221 3982.4038
4526 576.0682 4025.4265
4528 936.0487 4675.2071
4529 1111.7078 5553.5026
4532 1113.0978 5560.4526
4533 1114.5087 5567.5071
4534 1114.7057 5568.4921
4535 1114.9036 5569.4816
4536 929.2572 5569.4995
4537 1116.2983 5576.4551
4538 930.6548 5577.8851
4539 1117.6956 5583.4416
4540 1118.1045 5585.4861
4541 1118.3085 5586.5061
4542 1119.1007 5590.4671
4543 933.0834 5592.4567
4544 1120.4937 5597.4321
4545 1120.8920 5599.4236
4546 1120.9067 5599.4971
4547 1121.1073 5600.5001
4548 1121.4999 5602.4631
4549 1122.2981 5606.4541
4550 1122.2991 5606.4591
4551 935.5816 5607.4459
4552 935.7495 5608.4533
4553 1122.8828 5609.3776
4554 1122.9120 5609.5236
4555 1123.3105 5611.5161
4556 1124.0927 5615.4271
4557 1124.2927 5616.4271
4558 1125.4930 5622.4286
4559 938.2466 5623.4359
4560 938.2474 5623.4407
4561 1125.6986 5623.4566
4562 938.7468 5626.4371
4563 1126.3091 5626.5091
4564 939.0781 5628.4249
4565 1127.0818 5630.3726
4566 1127.2888 5631.4076
4567 1127.6881 5633.4041
4568 1128.2872 5636.3996
4569 1128.4916 5637.4216
4570 1128.4973 5637.4501
4571 940.5829 5637.4537
4572 940.9058 5639.3911
4573 941.0775 5640.4213
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