MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4353 666.1254 2660.4725
4354 666.1570 2660.5989
4355 888.1345 2661.3817
4357 889.4359 2665.2859
4358 889.4468 2665.3186
4359 893.4694 2677.3864
4360 537.4989 2682.4581
4362 896.7627 2687.2663
4363 899.7768 2696.3086
4366 675.8940 2699.5469
4367 677.1334 2704.5045
4368 677.1664 2704.6365
4370 682.3124 2725.2205
4371 909.7791 2726.3155
4372 546.3032 2726.4796
4373 682.6583 2726.6041
4377 688.1378 2748.5221
4378 688.1730 2748.6629
4379 692.8307 2767.2937
4382 697.9070 2787.5989
4383 699.1500 2792.5709
4384 699.1797 2792.6897
4385 559.5453 2792.6901
4387 562.9240 2809.5836
4388 563.9180 2814.5536
4389 707.3755 2825.4729
4390 708.9135 2831.6249
4391 710.1567 2836.5977
4392 568.3502 2836.7146
4393 710.1861 2836.7153
4394 949.4835 2845.4287
4395 571.7285 2853.6061
4396 572.7186 2858.5566
4397 956.1227 2865.3463
4398 719.9196 2875.6493
4399 721.1926 2880.7413
4400 577.1559 2880.7431
4401 581.5236 2902.5816
4402 970.1544 2907.4414
4403 970.1562 2907.4468
4404 730.1271 2916.4793
4405 973.1686 2916.4840
4406 730.1286 2916.4853
4407 730.1296 2916.4893
4408 730.9263 2919.6761
4409 732.1991 2924.7673
4410 585.9617 2924.7721
4411 733.3620 2929.4189
4412 587.0905 2930.4161
4413 587.2912 2931.4196
4414 734.1267 2932.4777
4416 734.8771 2935.4793
4417 734.8788 2935.4861
4418 979.5044 2935.4914
4419 979.8223 2936.4451
4420 735.1296 2936.4893
4421 588.8974 2939.4506
4422 737.1294 2944.4885
4423 737.1299 2944.4905
4424 590.0861 2945.3941
4425 737.6025 2946.3809
4426 590.3339 2946.6331
4428 983.4814 2947.4224
4429 737.8820 2947.4989
4430 493.4120 2954.4283
4432 739.8636 2955.4253
4433 741.1255 2960.4729
4434 741.8792 2963.4877
4435 741.9335 2963.7049
4436 594.7673 2968.8001
4437 743.2118 2968.8181
4438 744.1150 2972.4309
4439 595.6944 2973.4356
4440 744.6170 2974.4389
4441 599.1399 2990.6631
4442 603.5718 3012.8226
4443 754.4661 3013.8353
4444 606.9493 3029.7101
4445 607.9443 3034.6851
4446 763.9456 3051.7533
4447 765.2247 3056.8697
4448 612.5775 3057.8511
4449 766.4361 3061.7153
4450 1025.5282 3073.5628
4451 770.6376 3078.5213
4452 616.7116 3078.5216
4453 616.7454 3078.6906
4454 616.7759 3078.8431
4455 1032.2397 3093.6973
4456 776.2308 3100.8941
4457 621.3839 3101.8831
4458 625.7787 3123.8571
4459 629.9878 3144.9026
4460 787.4890 3145.9269
4461 634.3604 3166.7656
4462 634.3845 3166.8861
4463 1061.8651 3182.5735
4464 796.6509 3182.5745
4465 532.3271 3187.9189
4466 638.7936 3188.9316
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