MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4236 616.6183 2462.4441
4237 494.6424 2468.1756
4238 620.8632 2479.4237
4241 622.0992 2484.3677
4242 622.1299 2484.4905
4243 829.7682 2486.2828
4244 830.0964 2487.2674
4245 830.7501 2489.2285
4246 830.7516 2489.2330
4247 832.0812 2493.2218
4248 832.0820 2493.2242
4249 832.0822 2493.2248
4252 836.0798 2505.2176
4253 502.2779 2506.3531
4254 627.6117 2506.4177
4255 627.6236 2506.4653
4258 839.0984 2514.2734
4259 629.8066 2515.1973
4260 839.4081 2515.2025
4262 840.0831 2517.2275
4264 631.0776 2520.2813
4265 505.2602 2521.2646
4266 505.2632 2521.2796
4267 631.5732 2522.2637
4268 841.7779 2522.3119
4269 631.8691 2523.4473
4270 632.0649 2524.2305
4271 842.4178 2524.2316
4272 505.8537 2524.2321
4273 632.8024 2527.1805
4274 506.4434 2527.1806
4275 633.1063 2528.3961
4276 844.1170 2529.3292
4277 633.8002 2531.1717
4278 633.8004 2531.1725
4279 844.7327 2531.1763
4281 508.4596 2537.2616
4282 511.0828 2550.3776
4283 638.6314 2550.4965
4284 511.6576 2553.2516
4286 640.5790 2558.2869
4287 854.1018 2559.2836
4288 854.1035 2559.2887
4289 854.1037 2559.2893
4290 854.1039 2559.2899
4291 854.1041 2559.2905
4292 854.1050 2559.2932
4293 855.4374 2563.2904
4294 856.4307 2566.2703
4296 642.8759 2567.4745
4297 644.1129 2572.4225
4298 644.1436 2572.5453
4299 859.4377 2575.2913
4300 644.8301 2575.2913
4301 859.4378 2575.2916
4302 859.7685 2576.2837
4303 859.7702 2576.2888
4304 517.2599 2581.2631
4305 861.4304 2581.2694
4306 646.5727 2582.2617
4307 863.4210 2587.2412
4308 863.4355 2587.2847
4309 864.0950 2589.2632
4310 648.5771 2590.2793
4311 864.7679 2591.2819
4312 864.7685 2591.2837
4313 648.8284 2591.2845
4314 519.8885 2594.4061
4315 649.6353 2594.5121
4316 866.7521 2597.2345
4317 520.4548 2597.2376
4318 650.3168 2597.2381
4319 650.3172 2597.2397
4321 866.7625 2597.2657
4322 867.0868 2598.2386
4324 868.7681 2603.2825
4325 869.1000 2604.2782
4326 869.4392 2605.2958
4327 653.8814 2611.4965
4328 523.6489 2613.2081
4330 872.0863 2613.2371
4331 523.6559 2613.2431
4332 872.0936 2613.2590
4333 654.5719 2614.2585
4334 655.1183 2616.4441
4335 874.0991 2619.2755
4336 874.4019 2620.1839
4337 874.4420 2620.3042
4338 656.3079 2621.2025
4339 877.4167 2629.2283
4343 879.1378 2634.3916
4344 659.8032 2635.1837
4345 528.0441 2635.1841
4346 879.4035 2635.1887
4347 880.0974 2637.2704
4348 880.1069 2637.2989
4349 528.6934 2638.4306
4350 660.6483 2638.5641
4351 662.3646 2645.4293
4352 664.8884 2655.5245
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