MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4116 724.7212 2171.1418
4117 543.8149 2171.2305
4118 725.0399 2172.0979
4119 545.0546 2176.1893
4120 726.4440 2176.3102
4121 545.0864 2176.3165
4122 546.5201 2182.0513
4123 732.1006 2193.2800
4124 733.4160 2197.2262
4125 550.3356 2197.3133
4126 550.5642 2198.2277
4127 735.0554 2202.1444
4130 737.3699 2209.0879
4132 554.8251 2215.2713
4133 556.0613 2220.2161
4134 556.0924 2220.3405
4135 741.1236 2220.3490
4136 559.8198 2235.2501
4137 746.7756 2237.3050
4138 748.4255 2242.2547
4139 561.5734 2242.2645
4141 752.0740 2253.2002
4142 565.3120 2257.2189
4143 565.5774 2258.2805
4144 565.8314 2259.2965
4146 567.0980 2264.3629
4147 568.2706 2269.0533
4149 761.4510 2281.3312
4150 572.3271 2285.2793
4151 763.1018 2286.2836
4152 572.5942 2286.3477
4156 576.8372 2303.3197
4157 578.0743 2308.2681
4158 578.1044 2308.3885
4159 578.7897 2311.1297
4160 776.1262 2325.3568
4161 777.4418 2329.3036
4162 583.5817 2330.2977
4163 583.6060 2330.3949
4164 780.0833 2337.2281
4165 780.4141 2338.2205
4167 587.8431 2347.3433
4168 589.0807 2352.2937
4169 589.1107 2352.4137
4170 785.4136 2353.2190
4171 787.4230 2359.2472
4172 790.4130 2368.2172
4175 791.4042 2371.1908
4176 792.4517 2374.3333
4177 594.5915 2374.3369
4178 594.6095 2374.4089
4179 1194.1389 2386.2632
4180 597.5733 2386.2641
4181 597.5734 2386.2645
4182 796.4296 2386.2670
4183 796.4304 2386.2694
4184 597.5752 2386.2717
4185 597.5753 2386.2721
4186 597.5758 2386.2741
4187 597.5761 2386.2753
4188 597.6113 2386.4161
4189 598.8497 2391.3697
4190 600.0696 2396.2493
4191 600.0870 2396.3189
4192 600.1202 2396.4517
4193 800.4161 2398.2265
4195 803.7441 2408.2105
4197 482.8553 2409.2401
4198 603.3177 2409.2417
4200 604.5723 2414.2601
4201 805.7609 2414.2609
4202 604.5725 2414.2609
4203 805.7626 2414.2660
4204 806.7567 2417.2483
4205 484.4662 2417.2946
4206 807.1270 2418.3592
4207 405.0428 2424.2131
4208 607.0609 2424.2145
4209 485.8502 2424.2146
4210 809.0798 2424.2176
4212 485.8511 2424.2191
4213 486.8522 2429.2246
4214 811.0830 2430.2272
4215 608.5645 2430.2289
4217 488.2543 2436.2351
4218 813.1229 2436.3469
4219 813.1302 2436.3688
4220 610.1072 2436.3997
4223 611.0932 2440.3437
4224 611.1234 2440.4645
4226 611.5747 2442.2697
4227 611.8311 2443.2953
4228 612.0790 2444.2869
4229 614.0583 2452.2041
4230 491.4482 2452.2046
4231 616.2950 2461.1509
4232 493.4393 2462.1601
4233 493.4727 2462.3271
4234 821.8017 2462.3833
4235 616.6098 2462.4101
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