MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3861 645.9360 1934.7862
3862 968.4377 1934.8608
3863 968.4388 1934.8630
3866 485.2023 1936.7801
3867 969.9333 1937.8520
3869 970.4316 1938.8486
3870 647.3100 1938.9082
3871 485.7348 1938.9101
3873 486.9601 1943.8113
3874 649.2775 1944.8107
3878 488.2662 1949.0357
3879 650.7089 1949.1049
3880 650.9290 1949.7652
3881 488.4486 1949.7653
3882 488.5327 1950.1017
3883 976.4337 1950.8528
3884 976.9404 1951.8662
3885 489.7673 1955.0401
3886 489.7701 1955.0513
3887 978.5629 1955.1112
3888 652.7345 1955.1817
3889 652.7346 1955.1820
3890 652.9345 1955.7817
3891 653.0438 1956.1096
3892 653.0518 1956.1336
3893 490.0550 1956.1909
3895 653.4029 1957.1869
3899 654.0113 1959.0121
3900 654.0117 1959.0133
3901 490.9575 1959.8009
3902 654.2755 1959.8047
3903 655.0300 1962.0682
3904 491.7248 1962.8701
3905 655.2982 1962.8728
3906 658.0111 1971.0115
3907 493.7613 1971.0161
3908 493.7797 1971.0897
3909 658.3870 1972.1392
3910 659.6819 1976.0239
3911 659.9415 1976.8027
3912 495.2080 1976.8029
3913 396.3781 1976.8541
3914 495.2811 1977.0953
3915 660.0393 1977.0961
3916 495.2975 1977.1609
3917 495.4717 1977.8577
3918 660.2937 1977.8593
3919 660.3672 1978.0798
3920 660.3968 1978.1686
3921 496.2520 1980.9789
3924 662.0297 1983.0673
3925 994.0078 1986.0010
3926 663.3362 1986.9868
3928 663.3405 1986.9997
3930 994.5124 1987.0102
3931 663.3444 1987.0114
3932 499.2733 1993.0641
3933 665.3856 1993.1350
3934 665.6938 1994.0596
3935 499.7891 1995.1273
3936 499.9891 1995.9273
3937 499.9966 1995.9573
3938 500.0110 1996.0149
3939 666.3463 1996.0171
3940 666.6593 1996.9561
3941 400.3987 1996.9571
3942 500.2466 1996.9573
3943 667.3438 1999.0096
3944 500.7769 1999.0785
3946 667.7147 2000.1223
3947 1001.0795 2000.1444
3948 667.7285 2000.1637
3950 667.7455 2000.2147
3951 501.0627 2000.2217
3952 668.0308 2001.0706
3953 668.0331 2001.0775
3954 668.0345 2001.0817
3955 501.4991 2001.9673
3956 668.3657 2002.0753
3957 501.5261 2002.0753
3958 1002.0463 2002.0780
3959 1002.0464 2002.0782
3960 668.3667 2002.0783
3961 668.3675 2002.0807
3962 668.3677 2002.0813
3963 668.3678 2002.0816
3964 1002.0482 2002.0818
3965 668.3683 2002.0831
3966 668.3685 2002.0837
3967 668.3686 2002.0840
3968 668.3686 2002.0840
3969 668.3688 2002.0846
3970 668.3691 2002.0855
3971 668.3724 2002.0954
3972 668.6648 2002.9726
3973 669.3693 2005.0861
3974 670.3474 2008.0204
3978 503.2619 2009.0185
3982 670.6846 2009.0320
3983 670.6853 2009.0341
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