MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3742 616.0131 1845.0175
3744 616.3177 1845.9313
3745 462.5110 1846.0149
3746 616.3699 1846.0879
3747 616.6757 1847.0053
3749 617.3169 1848.9289
3750 463.2400 1848.9309
3751 925.9910 1849.9674
3753 464.7487 1854.9657
3754 619.6266 1855.8580
3755 619.6589 1855.9549
3756 620.6353 1858.8841
3757 465.7291 1858.8873
3758 465.7350 1858.9109
3760 620.9783 1859.9131
3761 621.3345 1860.9817
3762 466.2533 1860.9841
3763 621.3584 1861.0534
3764 621.6725 1861.9957
3765 466.5193 1862.0481
3766 622.0069 1862.9989
3767 622.0084 1863.0034
3768 622.0491 1863.1255
3769 467.7569 1866.9985
3770 934.5372 1867.0598
3772 623.6945 1868.0617
3773 623.7028 1868.0866
3774 623.7185 1868.1337
3775 623.7191 1868.1355
3777 468.7358 1870.9141
3778 624.6472 1870.9198
3779 468.9879 1871.9225
3781 625.3450 1873.0132
3782 625.6306 1873.8700
3783 625.6316 1873.8730
3784 938.0764 1874.1382
3785 938.4441 1874.8736
3786 938.5641 1875.1136
3787 938.9384 1875.8622
3788 626.9634 1877.8684
3789 470.7283 1878.8841
3792 628.6834 1883.0284
3793 471.7647 1883.0297
3794 629.3733 1885.0981
3795 629.6298 1885.8676
3796 630.6862 1889.0368
3797 473.2673 1889.0401
3798 630.6895 1889.0467
3799 630.7133 1889.1181
3800 630.9631 1889.8675
3801 946.4421 1890.8696
3803 946.4432 1890.8718
3804 631.2985 1890.8737
3805 631.2985 1890.8737
3806 474.4595 1893.8089
3807 475.2177 1896.8417
3808 949.4351 1896.8556
3810 634.9634 1901.8684
3811 952.4431 1902.8716
3812 952.4441 1902.8736
3813 477.2578 1905.0021
3814 636.0305 1905.0697
3818 954.4390 1906.8634
3819 954.4398 1906.8650
3820 477.7797 1907.0897
3821 956.5504 1911.0862
3822 638.0601 1911.1585
3823 478.7971 1911.1593
3824 638.0607 1911.1603
3825 478.9624 1911.8205
3826 638.2942 1911.8608
3827 479.0121 1912.0193
3828 638.3683 1912.0831
3829 638.3747 1912.1023
3830 957.4165 1912.8184
3831 638.6140 1912.8202
3832 638.6147 1912.8223
3833 479.2662 1913.0357
3834 638.7045 1913.0917
3836 640.2842 1917.8308
3838 960.4379 1918.8612
3839 960.4403 1918.8660
3840 960.9433 1919.8720
3842 480.9827 1919.9017
3843 962.4354 1922.8562
3845 641.9603 1922.8591
3846 643.3591 1927.0555
3847 482.7729 1927.0625
3848 643.6038 1927.7896
3849 643.6130 1927.8172
3850 482.9619 1927.8185
3851 482.9620 1927.8189
3852 643.9472 1928.8198
3854 644.2823 1929.8251
3855 645.3560 1933.0462
3856 645.3632 1933.0678
3857 645.3633 1933.0681
3858 484.2749 1933.0705
3859 484.2925 1933.1409
3860 645.7217 1934.1433
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