MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3635 445.7440 1778.9469
3636 594.0316 1779.0730
3637 594.2579 1779.7519
3638 594.3125 1779.9157
3640 594.3410 1780.0012
3641 891.0108 1780.0070
3642 594.3475 1780.0207
3643 594.3535 1780.0387
3644 891.0454 1780.0762
3645 594.3666 1780.0780
3646 594.6782 1781.0128
3647 892.9163 1783.8180
3648 595.9938 1784.9596
3649 596.9962 1787.9668
3650 597.2928 1788.8566
3651 448.2215 1788.8569
3652 597.3333 1788.9781
3653 597.3347 1788.9823
3654 895.9970 1789.9794
3655 598.0374 1791.0904
3656 598.2354 1791.6844
3657 449.1793 1792.6881
3658 449.4504 1793.7725
3659 598.9315 1793.7727
3660 449.4633 1793.8241
3661 598.9487 1793.8243
3662 599.2982 1794.8728
3663 449.7501 1794.9713
3664 599.3320 1794.9742
3665 599.6898 1796.0476
3666 900.9688 1799.9230
3667 901.4855 1800.9564
3668 601.3365 1800.9877
3669 601.3368 1800.9886
3670 601.3593 1801.0561
3671 601.5763 1801.7071
3672 451.5042 1801.9877
3673 451.6851 1802.7113
3674 602.6639 1804.9699
3675 602.6661 1804.9765
3676 602.6664 1804.9774
3677 602.6666 1804.9780
3678 602.6713 1804.9921
3679 602.9975 1805.9707
3680 903.9937 1805.9728
3681 903.9944 1805.9742
3682 603.0006 1805.9800
3683 603.0007 1805.9803
3684 603.0013 1805.9821
3685 604.9809 1811.9209
3686 606.6562 1816.9468
3687 455.2461 1816.9553
3688 606.6834 1817.0284
3689 607.0008 1817.9806
3690 607.3724 1819.0954
3692 607.9966 1820.9680
3693 607.9984 1820.9734
3694 911.9910 1821.9674
3695 911.9933 1821.9720
3697 608.3317 1821.9733
3698 456.7499 1822.9705
3699 608.7079 1823.1019
3700 608.7094 1823.1064
3701 912.9599 1823.9052
3702 609.0167 1824.0283
3703 913.0248 1824.0350
3704 609.0280 1824.0622
3705 365.9884 1824.9056
3706 609.3535 1825.0387
3707 914.4813 1826.9480
3708 457.9918 1827.9381
3709 457.9929 1827.9425
3710 457.9931 1827.9433
3711 610.3249 1827.9529
3712 610.3254 1827.9544
3715 610.6692 1828.9858
3716 917.4905 1832.9664
3717 611.9972 1832.9698
3718 917.4934 1832.9722
3719 612.3303 1833.9691
3720 612.3327 1833.9763
3721 612.3328 1833.9766
3722 612.3328 1833.9766
3723 612.7118 1835.1136
3724 613.9634 1838.8684
3725 613.9752 1838.9038
3726 920.4737 1838.9328
3727 613.9849 1838.9329
3728 613.9868 1838.9386
3729 460.7576 1839.0013
3731 920.9417 1839.8688
3732 460.9833 1839.9041
3733 614.3610 1840.0612
3734 461.7366 1842.9173
3735 615.3140 1842.9202
3736 461.7377 1842.9217
3737 922.9663 1843.9180
3738 615.6487 1843.9243
3739 461.9890 1843.9269
3741 616.0119 1845.0139
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