MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3509 859.8948 1717.7750
3510 573.9893 1718.9461
3511 860.4954 1718.9762
3512 431.7287 1722.8857
3513 863.4189 1724.8232
3514 575.9492 1724.8258
3516 576.6129 1726.8169
3517 864.4213 1726.8280
3518 576.6339 1726.8799
3520 576.9926 1727.9560
3521 577.3079 1728.9019
3522 433.2352 1728.9117
3523 577.3317 1728.9733
3524 866.0199 1730.0252
3525 578.0211 1731.0415
3526 866.8811 1731.7476
3527 866.8998 1731.7850
3528 867.8945 1733.7744
3529 579.3134 1734.9184
3530 434.7370 1734.9189
3531 579.3328 1734.9766
3532 868.5304 1735.0462
3533 579.3563 1735.0471
3534 579.3578 1735.0516
3537 868.9976 1735.9806
3538 579.6701 1735.9885
3539 579.6783 1736.0131
3544 580.2609 1737.7609
3545 869.9393 1737.8640
3546 580.5898 1738.7476
3547 870.3851 1738.7556
3548 870.3922 1738.7698
3549 871.4155 1740.8164
3550 581.2802 1740.8188
3551 581.3170 1740.9292
3552 871.4879 1740.9612
3553 871.4903 1740.9660
3554 871.4920 1740.9694
3555 871.9116 1741.8086
3556 581.6104 1741.8094
3557 581.6631 1741.9675
3558 581.6639 1741.9699
3559 581.6643 1741.9711
3560 581.6646 1741.9720
3561 581.6666 1741.9780
3562 872.4139 1742.8132
3563 872.9011 1743.7876
3564 873.3905 1744.7664
3565 873.4874 1744.9602
3566 873.4876 1744.9606
3567 873.4887 1744.9628
3568 873.8903 1745.7660
3569 583.3612 1747.0618
3571 583.9387 1748.7943
3572 584.2680 1749.7822
3577 876.4645 1750.9144
3578 584.6464 1750.9174
3582 584.6562 1750.9468
3583 876.9650 1751.9154
3584 351.3907 1751.9171
3585 438.9880 1751.9229
3586 438.9881 1751.9233
3587 585.0098 1752.0076
3589 877.8733 1753.7320
3590 585.5862 1753.7368
3591 585.9143 1754.7211
3592 878.3680 1754.7214
3593 585.9172 1754.7298
3594 878.3735 1754.7324
3595 878.4148 1754.8150
3596 585.9520 1754.8342
3597 878.4244 1754.8342
3600 878.9203 1755.8260
3602 879.4191 1756.8236
3603 586.6485 1756.9237
3604 440.2383 1756.9241
3606 879.4717 1756.9288
3607 586.6611 1756.9615
3608 586.6837 1757.0293
3609 586.9961 1757.9665
3612 588.3278 1761.9616
3613 441.7006 1762.7733
3614 882.8778 1763.7410
3615 882.8862 1763.7578
3616 588.9277 1763.7613
3617 441.9479 1763.7625
3618 588.9335 1763.7787
3619 588.9961 1763.9665
3620 589.2615 1764.7627
3622 589.9693 1766.8861
3624 590.9060 1769.6962
3625 591.0045 1769.9917
3626 591.0050 1769.9932
3627 591.3370 1770.9892
3628 887.4521 1772.8896
3629 591.9834 1772.9284
3630 592.0045 1772.9917
3631 444.4897 1773.9297
3633 592.6496 1774.9270
3634 592.6967 1775.0683
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