MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3387 527.9806 1580.9200
3388 794.4429 1586.8712
3390 796.9456 1591.8766
3391 533.3028 1596.8866
3392 533.6177 1597.8313
3393 799.9822 1597.9498
3394 533.6592 1597.9558
3395 535.2864 1602.8374
3396 802.4572 1602.8998
3397 535.3282 1602.9628
3398 802.4907 1602.9668
3399 535.3297 1602.9673
3400 535.6404 1603.8994
3401 535.6530 1603.9372
3402 537.2910 1608.8512
3403 540.6299 1618.8679
3404 540.9861 1619.9365
3405 542.6349 1624.8829
3406 542.6349 1624.8829
3407 542.6565 1624.9477
3410 816.4550 1630.8954
3411 816.9294 1631.8442
3412 817.9146 1633.8146
3415 818.4078 1634.8010
3416 545.9413 1634.8021
3417 818.4121 1634.8096
3418 818.4172 1634.8198
3419 545.9478 1634.8216
3420 546.6395 1636.8967
3421 819.9506 1637.8866
3422 546.9700 1637.8882
3423 819.9515 1637.8884
3424 819.9575 1637.9004
3425 411.2195 1640.8489
3427 821.9951 1641.9756
3428 548.3361 1641.9865
3429 549.2875 1644.8407
3430 549.9616 1646.8630
3431 824.4708 1646.9270
3432 550.0047 1646.9923
3433 550.3167 1647.9283
3434 550.3171 1647.9295
3435 550.3251 1647.9535
3436 825.0052 1647.9958
3437 550.6277 1648.8613
3438 551.9672 1652.8798
3439 554.0093 1659.0061
3444 555.3052 1662.8938
3445 832.9072 1663.7998
3446 555.6619 1663.9639
3448 833.9113 1665.8080
3449 834.4019 1666.7892
3450 557.3102 1668.9088
3451 557.3111 1668.9115
3452 835.9090 1669.8034
3453 557.6675 1669.9807
3455 418.9486 1671.7653
3456 558.2625 1671.7657
3457 558.5903 1672.7491
3458 419.4454 1673.7525
3460 559.2887 1674.8443
3461 559.3138 1674.9196
3462 559.9534 1676.8384
3463 559.9603 1676.8591
3464 560.2930 1677.8572
3465 422.2255 1684.8729
3466 562.6336 1684.8790
3467 562.6572 1684.9498
3468 844.0067 1685.9988
3469 563.3467 1687.0183
3471 564.6371 1690.8895
3472 846.5165 1691.0184
3473 846.9845 1691.9544
3474 564.9934 1691.9584
3475 564.9948 1691.9626
3476 565.0027 1691.9863
3477 565.0159 1692.0259
3480 566.6425 1696.9057
3481 850.8901 1699.7656
3482 852.4785 1702.9424
3483 569.9813 1706.9221
3484 570.3376 1707.9910
3485 570.6685 1708.9837
3486 855.5206 1709.0266
3488 571.9852 1712.9338
3489 571.9949 1712.9629
3490 429.2483 1712.9641
3491 857.4897 1712.9648
3492 857.4901 1712.9656
3493 571.9959 1712.9659
3494 857.4919 1712.9692
3495 857.4931 1712.9716
3496 571.9983 1712.9731
3498 572.3313 1713.9721
3499 572.3315 1713.9727
3503 572.3328 1713.9766
3505 572.3350 1713.9832
3506 858.9022 1715.7898
3507 859.3981 1716.7816
3508 573.3508 1717.0306
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