MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3278 365.1580 1456.6029
3279 486.8750 1457.6032
3280 730.3643 1458.7140
3281 730.3663 1458.7180
3282 731.3724 1460.7302
3283 489.2304 1464.6694
3284 367.1749 1464.6705
3286 367.1753 1464.6721
3287 489.2318 1464.6736
3288 489.2758 1464.8056
3289 733.9428 1465.8710
3290 489.9273 1466.7601
3291 734.8356 1467.6566
3292 491.2355 1470.6847
3293 736.3503 1470.6860
3295 491.2601 1470.7585
3296 736.4185 1470.8224
3297 491.2822 1470.8248
3298 736.4500 1470.8854
3299 491.3029 1470.8869
3300 491.6135 1471.8187
3301 737.3347 1472.6548
3303 493.2653 1476.7741
3304 370.9160 1479.6349
3305 741.3275 1480.6404
3306 494.5545 1480.6417
3307 495.5885 1483.7437
3308 742.8804 1483.7462
3310 495.5905 1483.7497
3311 496.6032 1486.7878
3312 372.9176 1487.6413
3313 744.8314 1487.6482
3314 496.9576 1487.8510
3315 497.5556 1489.6450
3317 498.6089 1492.8049
3318 498.6090 1492.8052
3319 747.4402 1492.8658
3320 498.6298 1492.8676
3321 748.3237 1494.6328
3322 750.4156 1498.8166
3323 751.8154 1501.6162
3324 376.6613 1502.6161
3326 501.8796 1502.6170
3327 754.4076 1506.8006
3328 755.3250 1508.6354
3329 503.8858 1508.6356
3330 503.9528 1508.8366
3331 504.3054 1509.8944
3332 755.9558 1509.8970
3333 756.3087 1510.6028
3334 505.9349 1514.7829
3335 505.9566 1514.8480
3336 758.4317 1514.8488
3337 758.4635 1514.9124
3338 505.9784 1514.9134
3339 506.2474 1515.7204
3340 506.2886 1515.8440
3341 507.9407 1520.8003
3342 508.2886 1521.8440
3343 764.4142 1526.8138
3344 765.3143 1528.6140
3345 765.8353 1529.6560
3346 511.2353 1530.6841
3347 511.2791 1530.8155
3348 511.6080 1531.8022
3349 511.6331 1531.8775
3350 768.9070 1535.7994
3351 512.9408 1535.8006
3353 513.2839 1536.8299
3354 513.2859 1536.8359
3355 513.3056 1536.8950
3356 770.9352 1539.8558
3357 515.2714 1542.7924
3358 772.4302 1542.8458
3359 774.8400 1547.6654
3361 518.6072 1552.7998
3362 518.6273 1552.8601
3363 777.9674 1553.9202
3364 520.2669 1557.7789
3365 779.8976 1557.7806
3366 520.6110 1558.8112
3367 780.4455 1558.8764
3368 520.6328 1558.8766
3369 780.4759 1558.9372
3370 520.6538 1558.9396
3371 520.6539 1558.9399
3372 780.4840 1558.9534
3373 520.9660 1559.8762
3374 521.5862 1561.7368
3376 522.2884 1563.8434
3377 522.6159 1564.8259
3378 522.9749 1565.9029
3379 523.5424 1567.6054
3380 393.1520 1568.5789
3381 525.5899 1573.7479
3382 787.8813 1573.7480
3383 525.9544 1574.8414
3384 526.3096 1575.9070
3385 527.9596 1580.8570
3386 527.9607 1580.8603
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