MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3122 460.9062 1379.6968
3123 460.9198 1379.7376
3126 460.9213 1379.7421
3128 461.9087 1382.7043
3130 692.3870 1382.7594
3131 461.9290 1382.7652
3132 692.4230 1382.8314
3133 692.4233 1382.8320
3134 461.9513 1382.8321
3136 462.2625 1383.7657
3139 463.5777 1387.7113
3143 463.9141 1388.7205
3144 695.3828 1388.7510
3145 695.4000 1388.7854
3146 464.2402 1389.6988
3147 696.8601 1391.7056
3148 464.9193 1391.7361
3149 348.9419 1391.7385
3151 465.2330 1392.6772
3154 465.2523 1392.7351
3155 465.5129 1393.5169
3158 465.5849 1393.7329
3159 465.9078 1394.7016
3160 698.3668 1394.7190
3161 466.2513 1395.7321
3164 466.2515 1395.7327
3165 466.2516 1395.7330
3166 698.8746 1395.7346
3167 466.2526 1395.7360
3168 698.8754 1395.7362
3169 466.2527 1395.7363
3172 467.2512 1398.7318
3173 467.6053 1399.7941
3174 468.9021 1403.6845
3175 468.9026 1403.6860
3179 469.2567 1404.7483
3180 469.2576 1404.7510
3181 469.2780 1404.8122
3182 703.4141 1404.8136
3183 469.5661 1405.6765
3184 703.9125 1405.8104
3185 470.2520 1407.7342
3186 470.2528 1407.7366
3187 470.5784 1408.7134
3188 353.1927 1408.7417
3189 353.1935 1408.7449
3190 470.9090 1409.7052
3191 706.3586 1410.7026
3192 706.3898 1410.7650
3193 471.5743 1411.7011
3195 706.9018 1411.7890
3196 709.8881 1417.7616
3197 710.8356 1419.6566
3199 355.9217 1419.6577
3201 474.5808 1420.7206
3202 474.5941 1420.7605
3204 474.5961 1420.7665
3205 474.6007 1420.7803
3206 711.9285 1421.8424
3207 712.3872 1422.7598
3208 357.4208 1425.6541
3209 476.2256 1425.6550
3210 713.8423 1425.6700
3211 713.8521 1425.6896
3212 713.8903 1425.7660
3225 476.5834 1426.7284
3227 476.5837 1426.7293
3229 476.5838 1426.7296
3230 476.5838 1426.7296
3235 476.6055 1426.7947
3236 714.4047 1426.7948
3237 714.4368 1426.8590
3238 476.6271 1426.8595
3239 714.4395 1426.8644
3240 357.9193 1427.6481
3241 476.9142 1427.7208
3242 476.9157 1427.7253
3243 476.9382 1427.7928
3244 715.4268 1428.8390
3245 478.2291 1431.6655
3247 716.8506 1431.6866
3249 478.9019 1433.6839
3251 718.8943 1435.7740
3252 361.4154 1441.6325
3253 481.5516 1441.6330
3255 481.5895 1441.7467
3256 481.5904 1441.7494
3257 481.9275 1442.7607
3258 482.2818 1443.8236
3259 483.5576 1447.6510
3263 483.9328 1448.7766
3264 483.9333 1448.7781
3265 725.4271 1448.8396
3266 483.9545 1448.8417
3267 725.9257 1449.8368
3269 726.3572 1450.6998
3271 485.1709 1452.4909
3272 485.2542 1452.7408
3273 485.2985 1452.8737
3276 728.4037 1454.7928
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