MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2995 662.3346 1322.6546
2999 662.3359 1322.6572
3001 662.3634 1322.7122
3002 662.3951 1322.7756
3003 662.7932 1323.5718
3004 662.7947 1323.5748
3005 662.7974 1323.5802
3006 443.5029 1327.4869
3007 443.8314 1328.4724
3008 665.2437 1328.4728
3011 665.3424 1328.6702
3012 666.2974 1330.5802
3013 666.3580 1330.7014
3014 667.2686 1332.5226
3015 445.1822 1332.5248
3016 667.8995 1333.7844
3017 445.8835 1334.6287
3021 447.1815 1338.5227
3022 670.2731 1338.5316
3023 670.3188 1338.6230
3025 447.2351 1338.6835
3026 447.2528 1338.7366
3027 670.3760 1338.7374
3028 670.3773 1338.7400
3029 447.2742 1338.8008
3030 670.4089 1338.8032
3031 670.4092 1338.8038
3032 670.7940 1339.5734
3033 670.7944 1339.5742
3035 337.1628 1344.6221
3036 673.3237 1344.6328
3037 449.2383 1344.6931
3038 673.3866 1344.7586
3040 337.4405 1345.7329
3043 337.4412 1345.7357
3045 450.1853 1347.5341
3046 451.1939 1350.5599
3047 676.3221 1350.6296
3049 677.2864 1352.5582
3050 452.5072 1354.4998
3051 678.2581 1354.5016
3052 452.5750 1354.7032
3053 452.5773 1354.7101
3054 678.7910 1355.5674
3055 678.7911 1355.5676
3056 452.9293 1355.7661
3058 681.2649 1360.5152
3059 454.5134 1360.5184
3060 454.5409 1360.6009
3061 681.3096 1360.6046
3063 454.5819 1360.7239
3064 341.1942 1360.7477
3065 681.3995 1360.7844
3066 454.6021 1360.7845
3067 341.3878 1361.5221
3068 454.8483 1361.5231
3069 341.4398 1361.7301
3070 681.8990 1361.7834
3071 455.2461 1362.7165
3073 455.5875 1363.7407
3074 341.9426 1363.7413
3075 455.5878 1363.7416
3077 682.8786 1363.7426
3078 341.9430 1363.7429
3079 455.5886 1363.7440
3081 455.5890 1363.7452
3082 455.5891 1363.7455
3084 682.8803 1363.7460
3085 341.9438 1363.7461
3086 341.9439 1363.7465
3087 341.9439 1363.7465
3088 455.5896 1363.7470
3089 455.5896 1363.7470
3090 455.5899 1363.7479
3093 683.8760 1365.7374
3094 683.8775 1365.7404
3095 456.2542 1365.7408
3096 683.8786 1365.7426
3097 683.8791 1365.7436
3098 683.8795 1365.7444
3099 456.5469 1366.6189
3100 684.3172 1366.6198
3101 684.3752 1366.7358
3102 684.3765 1366.7384
3103 456.9209 1367.7409
3104 342.9425 1367.7409
3105 685.2706 1368.5266
3106 457.1839 1368.5299
3107 685.8001 1369.5856
3108 457.5587 1369.6543
3109 457.8300 1370.4682
3110 458.2475 1371.7207
3111 459.5763 1375.7071
3112 459.5776 1375.7110
3113 459.8636 1376.5690
3114 459.9243 1376.7511
3115 460.1812 1377.5218
3117 345.4385 1377.7249
3119 689.9128 1377.8110
3121 345.9314 1379.6965
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