MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2847 418.5350 1252.5832
2856 628.3636 1254.7126
2857 419.5085 1255.5037
2860 629.2752 1256.5358
2861 419.8530 1256.5372
2862 315.1416 1256.5373
2864 315.1430 1256.5429
2865 419.8799 1256.6179
2869 629.3591 1256.7036
2871 421.1842 1260.5308
2872 421.1847 1260.5323
2874 421.8361 1262.4865
2875 632.2544 1262.4942
2876 422.4994 1264.4764
2878 633.7942 1265.5738
2879 423.2390 1266.6952
2880 634.3550 1266.6954
2882 423.5232 1267.5478
2883 634.8484 1267.6822
2886 424.5564 1270.6474
2888 425.1775 1272.5107
2889 425.2326 1272.6760
2890 637.3721 1272.7296
2891 425.5094 1273.5064
2892 637.8729 1273.7312
2893 319.6785 1274.6849
2894 425.9024 1274.6854
2895 638.3555 1274.6964
2896 638.8209 1275.6272
2897 426.5658 1276.6756
2898 427.1625 1278.4657
2899 640.2410 1278.4674
2901 640.3107 1278.6068
2902 640.3110 1278.6074
2905 640.3119 1278.6092
2906 640.3120 1278.6094
2907 640.3124 1278.6102
2908 640.3124 1278.6102
2909 640.3129 1278.6112
2911 640.3133 1278.6120
2912 640.3135 1278.6124
2913 640.3137 1278.6128
2914 640.3147 1278.6148
2915 640.3497 1278.6848
2916 640.3499 1278.6852
2917 640.3814 1278.7482
2918 427.5156 1279.5250
2919 640.8066 1279.5986
2920 640.8110 1279.6074
2921 427.8390 1280.4952
2922 641.3641 1280.7136
2923 429.1847 1284.5323
2926 643.3595 1284.7044
2927 643.3602 1284.7058
2929 643.3615 1284.7084
2931 643.7946 1285.5746
2932 644.2701 1286.5256
2933 429.8497 1286.5273
2934 644.3608 1286.7070
2935 430.8336 1289.4790
2936 645.8855 1289.7564
2937 431.2278 1290.6616
2938 646.3392 1290.6638
2939 323.6733 1290.6641
2941 646.8398 1291.6650
2943 324.1720 1292.6589
2944 432.2280 1293.6622
2945 432.5017 1294.4833
2946 432.5774 1294.7104
2947 648.3644 1294.7142
2948 648.3951 1294.7756
2949 648.3962 1294.7778
2950 649.3466 1296.6786
2951 434.1842 1299.5308
2952 434.5075 1300.5007
2953 434.5323 1300.5751
2954 651.3004 1300.5862
2955 434.5614 1300.6624
2956 434.5630 1300.6672
2957 651.3718 1300.7290
2958 651.8021 1301.5896
2961 435.1957 1302.5653
2966 653.8426 1305.6706
2968 654.3130 1306.6114
2970 327.6766 1306.6773
2973 656.3334 1310.6522
2974 437.8985 1310.6737
2975 657.3495 1312.6844
2976 657.3502 1312.6858
2979 657.8259 1313.6372
2980 657.8279 1313.6412
2981 439.1979 1314.5719
2983 659.2836 1316.5526
2987 439.9044 1316.6914
2988 439.9262 1316.7568
2989 659.3857 1316.7568
2990 659.8857 1317.7568
2991 440.8618 1319.5636
2992 441.8643 1322.5711
2993 441.8783 1322.6131
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