MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2683 560.3417 1118.6688
2685 374.8726 1121.5960
2686 562.2600 1122.5054
2687 375.4815 1123.4227
2688 562.7192 1123.4238
2690 375.5432 1123.6078
2691 1131.5281 1130.5208
2692 566.7770 1131.5394
2693 567.2776 1132.5406
2695 567.3218 1132.6290
2696 378.7994 1133.3764
2697 567.8019 1133.5892
2698 379.1977 1134.5713
2699 380.2050 1137.5932
2700 380.8738 1139.5996
2701 571.3332 1140.6518
2702 381.8816 1142.6230
2703 381.8822 1142.6248
2707 382.5430 1144.6072
2708 573.7229 1145.4312
2709 573.8253 1145.6360
2710 574.3083 1146.6020
2711 576.3069 1150.5992
2713 577.3243 1152.6340
2714 579.3162 1156.6178
2715 579.8464 1157.6782
2722 581.7972 1161.5798
2723 582.3241 1162.6336
2725 582.3561 1162.6976
2726 582.3563 1162.6980
2728 585.0606 1168.1066
2730 586.3116 1170.6086
2731 586.3132 1170.6118
2735 393.8736 1178.5990
2738 395.2054 1182.5944
2739 592.3137 1182.6128
2742 593.2877 1184.5608
2745 593.3469 1184.6792
2746 593.8444 1185.6742
2747 397.2167 1188.6283
2750 596.3223 1190.6300
2753 398.8933 1193.6581
2754 598.8232 1195.6318
2755 599.2980 1196.5814
2760 600.2968 1198.5790
2761 300.6526 1198.5813
2762 400.5344 1198.5814
2763 400.5347 1198.5823
2764 400.8663 1199.5771
2765 601.3303 1200.6460
2768 601.8598 1201.7050
2769 401.8639 1202.5699
2772 401.8651 1202.5735
2775 602.7950 1203.5754
2776 603.3054 1204.5962
2777 604.3380 1206.6614
2778 604.3700 1206.7254
2779 403.5393 1207.5961
2780 605.8110 1209.6074
2782 607.3430 1212.6714
2783 405.8571 1214.5495
2790 610.3047 1218.5948
2791 407.5354 1219.5844
2793 610.8754 1219.7362
2795 408.5493 1222.6261
2796 612.8206 1223.6266
2798 409.5230 1225.5472
2799 409.5436 1225.6090
2800 613.8120 1225.6094
2802 614.2702 1226.5258
2803 409.8509 1226.5309
2805 410.5551 1228.6435
2806 615.3601 1228.7056
2807 615.8000 1229.5854
2809 615.8589 1229.7032
2810 616.2922 1230.5698
2811 616.3364 1230.6582
2813 618.2886 1234.5626
2814 618.3362 1234.6578
2815 618.3363 1234.6580
2816 618.7879 1235.5612
2818 309.8980 1235.5629
2822 620.7741 1239.5336
2823 414.1857 1239.5353
2826 414.5136 1240.5190
2827 414.5269 1240.5589
2829 621.8310 1241.6474
2830 415.1883 1242.5431
2831 622.2808 1242.5470
2832 415.1904 1242.5494
2833 622.3358 1242.6570
2836 623.8738 1245.7330
2837 416.5193 1246.5361
2839 625.8419 1249.6692
2840 417.9027 1250.6863
2841 626.3515 1250.6884
2842 626.3807 1250.7468
2843 626.3826 1250.7506
2844 626.7714 1251.5282
2846 418.2129 1251.6169
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