MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:08:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 3980 |
670.6840 | 2009.0302 | 2008.9966 | 16.7 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
ASNGLNIHLNHYESLLGR + 2 Deamidated (NQ) |
| 2770 |
602.2933 | 1202.5720 | 1202.5832 | -9.30 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
QAWISEWQR |
| 2964 |
327.1675 | 1304.6409 | 1304.6361 | 3.69 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
WQSTSVNDVLR + Deamidated (NQ) |
| 3041 |
337.4408 | 1345.7341 | 1345.7606 | -19.7 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
VYPLTVGDKVQK |
| 3519 |
864.4838 | 1726.9530 | 1726.9577 | -2.70 | 1 | 4 | 1 | 1Score > 17 indicates identity |
LNALEGKLQTGEISVR |
| 2824 |
621.2666 | 1240.5186 | 1240.5207 | -1.68 | 0 | 4 | 0.4 | 1Score > 13 indicates identity |
TEQQYENATR + 2 Deamidated (NQ) |
| 2881 |
634.3641 | 1266.7136 | 1266.6932 | 16.1 | 0 | 4 | 0.46 | 1Score > 13 indicates identity |
TPVALLDAPASGR |
| 2781 |
606.8049 | 1211.5952 | 1211.6186 | -19.3 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
QLGVSYFLER + Deamidated (NQ) |
| 3601 |
878.9440 | 1755.8734 | 1755.9050 | -18.0 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
VQQGRLVVNGASMPQR + Deamidated (NQ); Oxidation (M) |
| 3044 |
337.4412 | 1345.7357 | 1345.7136 | 16.4 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
MSELIVSRQQR |
| 2287 |
414.2058 | 826.3970 | 826.3933 | 4.54 | 1 | 4 | 0.92 | 1Score > 16 indicates identity |
EKHQQR + 2 Deamidated (NQ) |
| 2343 |
893.4508 | 892.4435 | 892.4403 | 3.66 | 1 | 4 | 1.3 | 1Score > 18 indicates identity |
AAFEDKGR |
| 2741 |
395.8608 | 1184.5606 | 1184.5397 | 17.6 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
HCVWEEVAR |
| 3125 |
345.9425 | 1379.7409 | 1379.7483 | -5.35 | 0 | 4 | 0.86 | 1Score > 16 indicates identity |
IIFCGTLTAGSLK |
| 3872 |
485.9830 | 1939.9029 | 1939.9244 | -11.1 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
HLQQMQHSLGMTVGTVR + 2 Deamidated (NQ); Oxidation (M) |
| 2178 |
354.1964 | 706.3782 | 706.3796 | -1.88 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LRSGMK + Oxidation (M) |
| 3611 |
881.4874 | 1760.9602 | 1760.9308 | 16.7 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LLQDADVFITNVREK + Deamidated (NQ) |
| 4076 |
695.0237 | 2082.0493 | 2082.0527 | -1.66 | 1 | 4 | 1.9 | 1Score > 19 indicates identity |
MNSIEQALKIAEQHNISR + Deamidated (NQ) |
| 2661 |
548.7223 | 1095.4300 | 1095.4469 | -15.4 | 1 | 4 | 0.41 | 1Score > 13 indicates identity |
DFEDKDDGR |
| 4361 |
895.4604 | 2683.3594 | 2683.3470 | 4.60 | 0 | 4 | 1.6 | 1Score > 18 indicates identity |
ALAQAMHQAGKPLGFMCIAPAMLPK + 2 Oxidation (M) |
| 4251 |
835.0648 | 2502.1726 | 2502.2060 | -13.4 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
SIIPSGYGVNPEGPTQECLSALGR + Deamidated (NQ) |
| 2280 |
822.4517 | 821.4444 | 821.4508 | -7.71 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
QRLSYR |
| 2767 |
401.5365 | 1201.5877 | 1201.5938 | -5.12 | 1 | 4 | 2 | 1Score > 19 indicates identity |
AEQAALQADKR + 2 Deamidated (NQ) |
| 2358 |
302.8324 | 905.4754 | 905.4607 | 16.2 | 0 | 4 | 2 | 1Score > 19 indicates identity |
QAGKPYDK |
| 2002 |
543.3621 | 542.3548 | 542.3540 | 1.51 | 0 | 4 | 1.8 | 1Score > 19 indicates identity |
AAIIR |
| 2317 |
435.7578 | 869.5010 | 869.5083 | -8.31 | 1 | 4 | 0.64 | 1Score > 14 indicates identity |
ERLLSPR |
| 4014 |
509.0045 | 2031.9889 | 2031.9709 | 8.88 | 1 | 4 | 0.54 | 1Score > 20 indicates identity Score > 13 indicates homology |
AEPTSGGGQLSTAQKQNISR + 3 Deamidated (NQ) |
| 3156 |
465.5809 | 1393.7209 | 1393.7388 | -12.8 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
LGERSCAYVVLK |
| 3447 |
555.9594 | 1664.8564 | 1664.8774 | -12.6 | 1 | 4 | 1.9 | 1Score > 19 indicates identity |
AQVAIFKEIFDQVR + 2 Deamidated (NQ) |
| 3413 |
545.6299 | 1633.8679 | 1633.8498 | 11.1 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
FTSLGPLMDKLGNGGK |
| 2372 |
459.7207 | 917.4268 | 917.4202 | 7.20 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
EANEGDKR |
| 4070 |
519.7497 | 2074.9697 | 2075.0106 | -19.7 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
RVGETAWAQDTIADCLLR + Deamidated (NQ) |
| 2390 |
310.1582 | 927.4528 | 927.4661 | -14.4 | 0 | 3 | 0.8 | 1Score > 15 indicates identity |
GLLPENER + Deamidated (NQ) |
| 2507 |
494.7587 | 987.5028 | 987.4872 | 15.8 | 0 | 3 | 2.2 | 1Score > 19 indicates identity |
ELALEEER |
| 3991 |
673.6527 | 2017.9363 | 2017.9626 | -13.1 | 0 | 3 | 1.6 | 1Score > 18 indicates identity |
IDVSQVDVSMNGIELQDR + Deamidated (NQ) |
| 3999 |
675.3585 | 2023.0537 | 2023.0442 | 4.67 | 1 | 3 | 1.5 | 1Score > 18 indicates identity |
VMAGLGIAVVSTSKGVMTDR + 2 Oxidation (M) |
| 2389 |
310.1582 | 927.4528 | 927.4661 | -14.4 | 0 | 3 | 0.8 | 1Score > 15 indicates identity |
GLLPENER + Deamidated (NQ) |
| 2737 |
394.2089 | 1179.6049 | 1179.6261 | -18.0 | 1 | 3 | 1.5 | 1Score > 18 indicates identity |
DHRNWLALR |
| 3898 |
980.0395 | 1958.0644 | 1958.0320 | 16.6 | 0 | 3 | 1.2 | 1Score > 17 indicates identity |
NNTTLLSAITLEAALQQR + 2 Deamidated (NQ) |
| 3231 |
476.5841 | 1426.7305 | 1426.7052 | 17.7 | 0 | 3 | 2.2 | 1Score > 19 indicates identity |
LQQIEPNAGTEAR + Deamidated (NQ) |
| 3535 |
868.9810 | 1735.9474 | 1735.9468 | 0.35 | 1 | 3 | 1 | 1Score > 16 indicates identity |
IVTEEVVVKNHNAVGK + Deamidated (NQ) |
| 3632 |
444.7264 | 1774.8765 | 1774.9036 | -15.3 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
SGITLPTREMWAQLR + Deamidated (NQ); Oxidation (M) |
| 4145 |
567.0674 | 2264.2405 | 2264.2092 | 13.8 | 0 | 3 | 0.82 | 1Score > 15 indicates identity |
SYPITIASGLFNEPASFLPLK |
| 2623 |
356.1640 | 1065.4702 | 1065.4761 | -5.53 | 0 | 3 | 0.82 | 1Score > 15 indicates identity |
TMLQQQGSR + 2 Deamidated (NQ); Oxidation (M) |
| 2078 |
322.1648 | 642.3150 | 642.3085 | 10.1 | 0 | 3 | 0.48 | 1Score > 13 indicates identity |
HTTER |
| 2509 |
496.7747 | 991.5348 | 991.5161 | 19.0 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
IPAYEMIR |
| 3153 |
349.1903 | 1392.7321 | 1392.7136 | 13.3 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
EDVQAYVKEAIK + Deamidated (NQ) |
| 3541 |
435.2404 | 1736.9325 | 1736.9420 | -5.50 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
VNQPQDLGIALARAIR + 3 Deamidated (NQ) |
| 2610 |
527.8055 | 1053.5964 | 1053.5818 | 13.9 | 1 | 3 | 0.65 | 1Score > 14 indicates identity |
ANNKVGLAPVA + Deamidated (NQ) |
| 3574 |
438.7353 | 1750.9121 | 1750.9254 | -7.58 | 1 | 3 | 1.6 | 1Score > 18 indicates identity |
AFKDLGFDSLAAVELR |
| 4060 |
688.3211 | 2061.9415 | 2061.9347 | 3.30 | 1 | 3 | 1.9 | 1Score > 19 indicates identity |
MSSMTTTDNKAFLNELAR + Deamidated (NQ); 2 Oxidation (M) |
| 3989 |
673.0214 | 2016.0424 | 2016.0713 | -14.4 | 1 | 3 | 1.9 | 1Score > 18 indicates identity |
NNLPLLNDLRVFMLVAR + 3 Deamidated (NQ); Oxidation (M) |
| 3500 |
857.9937 | 1713.9728 | 1713.9625 | 6.05 | 1 | 3 | 0.59 | 1Score > 13 indicates identity |
GVELLSTGGTARLLAEK |
| 4051 |
514.7552 | 2054.9917 | 2054.9612 | 14.8 | 1 | 3 | 2.5 | 1Score > 20 indicates identity |
TAMSSNIELASAEVMKQGR + Deamidated (NQ); 2 Oxidation (M) |
| 3927 |
994.5030 | 1986.9914 | 1987.0295 | -19.2 | 0 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
TLTIAAPGAASMQLAENALK + Deamidated (NQ); Oxidation (M) |
| 2903 |
640.3117 | 1278.6088 | 1278.5948 | 11.0 | 0 | 3 | 1.9 | 1Score > 18 indicates identity |
MMQLTGNPEVK + 2 Oxidation (M) |
| 4166 |
782.0927 | 2343.2563 | 2343.3022 | -19.6 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
NIALQAAQPLVHGVSLTLQRGR + 2 Deamidated (NQ) |
| 4016 |
509.2581 | 2033.0033 | 2032.9710 | 15.9 | 1 | 3 | 2.4 | 1Score > 19 indicates identity |
QDAVQHAQQLLKMMGYGV + Deamidated (NQ); Oxidation (M) |
| 4050 |
1028.0333 | 2054.0520 | 2054.0756 | -11.5 | 1 | 3 | 2 | 1Score > 18 indicates identity |
QTQTPRNLSIISPTGLGDR + Deamidated (NQ) |
| 3234 |
476.5844 | 1426.7314 | 1426.7052 | 18.4 | 0 | 3 | 2.4 | 1Score > 19 indicates identity |
LQQIEPNAGTEAR + Deamidated (NQ) |
| 3218 |
476.5821 | 1426.7245 | 1426.7092 | 10.7 | 0 | 3 | 2.4 | 1Score > 19 indicates identity |
LGIPDEALHNYGK + Deamidated (NQ) |
| 4199 |
805.7601 | 2414.2585 | 2414.2378 | 8.57 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
FVEGKPLMLEDSYMPVKLFR + Oxidation (M) |
| 2729 |
585.7927 | 1169.5708 | 1169.5863 | -13.2 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
LQLANVSCHK + Deamidated (NQ) |
| 3010 |
443.8973 | 1328.6701 | 1328.6738 | -2.78 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
YQKINAHHYR |
| 2845 |
418.1851 | 1251.5335 | 1251.5441 | -8.50 | 0 | 3 | 0.6 | 1Score > 13 indicates identity |
MFEINPVNNR + 3 Deamidated (NQ); Oxidation (M) |
| 3057 |
340.9355 | 1359.7129 | 1359.7068 | 4.49 | 0 | 3 | 0.93 | 1Score > 20 indicates identity Score > 15 indicates homology |
MQLEVVDILER + Oxidation (M) |
| 3581 |
438.7429 | 1750.9425 | 1750.9254 | 9.78 | 1 | 3 | 1.3 | 1Score > 16 indicates identity |
AFKDLGFDSLAAVELR |
| 2868 |
419.8875 | 1256.6407 | 1256.6513 | -8.46 | 0 | 3 | 1.9 | 1Score > 18 indicates identity |
IAIAVGNADAWR + Deamidated (NQ) |
| 2283 |
412.2290 | 822.4434 | 822.4422 | 1.56 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
LKEFMR |
| 2734 |
393.5333 | 1177.5781 | 1177.5615 | 14.1 | 0 | 3 | 0.94 | 1Score > 20 indicates identity Score > 15 indicates homology |
LNIDDVDFAR + Deamidated (NQ) |
| 3232 |
476.5841 | 1426.7305 | 1426.7052 | 17.7 | 0 | 3 | 2.5 | 1Score > 19 indicates identity |
LQQIEPNAGTEAR + Deamidated (NQ) |
| 2113 |
333.1911 | 664.3676 | 664.3544 | 19.9 | 0 | 3 | 1.7 | 1Score > 17 indicates identity |
EIFTR |
| 3844 |
962.4358 | 1922.8570 | 1922.8462 | 5.65 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
EQGMEGARDLALEMANR + Deamidated (NQ); 2 Oxidation (M) |
| 4280 |
846.4172 | 2536.2298 | 2536.1870 | 16.9 | 0 | 3 | 2.5 | 1Score > 19 indicates identity |
NLNSIPAFDDNYIWVLNDEAGR + Deamidated (NQ) |
| 2848 |
418.5795 | 1252.7167 | 1252.7404 | -19.0 | 1 | 3 | 0.65 | 1Score > 13 indicates identity |
IVSVPLWQRR |
| 3479 |
565.9932 | 1694.9578 | 1694.9679 | -5.95 | 0 | 3 | 0.81 | 1Score > 14 indicates identity |
LLANRPESALAVTLAR + Deamidated (NQ) |
| 4173 |
593.0709 | 2368.2545 | 2368.2386 | 6.70 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
SLVEAAFSQLSAERSFASLSLR |
| 2538 |
337.8151 | 1010.4235 | 1010.4305 | -6.95 | 0 | 3 | 0.71 | 1Score > 14 indicates identity |
DGTFTNAER + Deamidated (NQ) |
| 3790 |
940.4628 | 1878.9110 | 1878.8782 | 17.5 | 0 | 3 | 2.2 | 1Score > 19 indicates identity |
ADCLNNVLDAITQFER + Deamidated (NQ) |
| 2925 |
429.2297 | 1284.6673 | 1284.6786 | -8.80 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
INGEIRAQEVR + Deamidated (NQ) |
| 2081 |
324.1737 | 646.3328 | 646.3398 | -10.8 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
NAGKTR + Deamidated (NQ) |
| 4148 |
758.7358 | 2273.1856 | 2273.1573 | 12.4 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
INKDDIVINDIAVSLSNICR + 2 Deamidated (NQ) |
| 2003 |
543.3629 | 542.3556 | 542.3540 | 2.99 | 0 | 3 | 2.3 | 1Score > 19 indicates identity |
AAIIR |
| 2117 |
670.4249 | 669.4176 | 669.4173 | 0.42 | 0 | 3 | 0.55 | 1Score > 13 indicates identity |
ATPLIR |
| 2940 |
431.2294 | 1290.6664 | 1290.6642 | 1.67 | 0 | 3 | 2.5 | 1Score > 19 indicates identity |
LGQGPGLMLFDK + Oxidation (M) |
| 3124 |
345.9418 | 1379.7381 | 1379.7483 | -7.38 | 0 | 2 | 1.2 | 1Score > 16 indicates identity |
IIFCGTLTAGSLK |
| 2752 |
596.3547 | 1190.6948 | 1190.7135 | -15.7 | 0 | 2 | 0.57 | 1Score > 13 indicates identity |
VALATTHLPLR |
| 4068 |
690.9865 | 2069.9377 | 2069.9728 | -17.0 | 1 | 2 | 1.8 | 1Score > 17 indicates identity |
VATNLARNMVTQWGFSEK + 3 Deamidated (NQ); Oxidation (M) |
| 3275 |
728.3678 | 1454.7210 | 1454.7412 | -13.9 | 1 | 2 | 2.2 | 1Score > 18 indicates identity |
GALQQEMTRIHR + Oxidation (M) |
| 2959 |
434.8768 | 1301.6086 | 1301.6285 | -15.3 | 0 | 2 | 1.4 | 1Score > 16 indicates identity |
GQSIDDMIPAQK |
| 4295 |
642.8259 | 2567.2745 | 2567.3119 | -14.6 | 1 | 2 | 2.9 | 1Score > 20 indicates identity |
TVDPLAGSYYIESLTDQIVKQAR + Deamidated (NQ) |
| 2655 |
543.8011 | 1085.5876 | 1085.6015 | -12.8 | 1 | 2 | 2.5 | 1Score > 19 indicates identity |
QVLNMPTKR |
| 4019 |
680.0150 | 2037.0232 | 2037.0275 | -2.10 | 0 | 2 | 2.8 | 1Score > 19 indicates identity |
MQTVMQTLLPYLNQALR + 2 Deamidated (NQ); Oxidation (M) |
| 3543 |
579.9857 | 1736.9353 | 1736.9097 | 14.7 | 1 | 2 | 1.4 | 1Score > 16 indicates identity |
QPVYQLYTLLNRAR + 3 Deamidated (NQ) |
| 3542 |
579.9849 | 1736.9329 | 1736.9097 | 13.4 | 1 | 2 | 1.6 | 1Score > 17 indicates identity |
QPVYQLYTLLNRAR + 3 Deamidated (NQ) |
| 3285 |
733.3430 | 1464.6714 | 1464.6692 | 1.53 | 0 | 2 | 1.7 | 1Score > 17 indicates identity |
GTSEISAGNADLSSR + Deamidated (NQ) |
| 3835 |
640.0194 | 1917.0364 | 1917.0095 | 14.0 | 1 | 2 | 1 | 1Score > 15 indicates identity |
QADGEKVIQVPIDLYTK + Deamidated (NQ) |
| 4323 |
868.7676 | 2603.2810 | 2603.2901 | -3.51 | 1 | 2 | 1 | 1Score > 20 indicates identity Score > 15 indicates homology |
GEEGLFDLLRELGPMTVEDLAQR + Oxidation (M) |
| 2510 |
496.7749 | 991.5352 | 991.5161 | 19.4 | 0 | 2 | 1.8 | 1Score > 17 indicates identity |
IPAYEMIR |
| 2740 |
395.8606 | 1184.5600 | 1184.5422 | 15.0 | 0 | 2 | 1.8 | 1Score > 17 indicates identity |
GDQAPDADQLR |
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