MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2554 509.2393 1016.4640
2556 1020.5394 1019.5321
2558 341.4886 1021.4440
2559 513.2769 1024.5392
2560 342.7068 1025.0986
2561 513.7318 1025.4490
2562 342.8237 1025.4493
2563 342.8239 1025.4499
2565 342.8242 1025.4508
2566 513.8083 1025.6020
2568 344.1682 1029.4828
2569 515.7490 1029.4834
2570 344.1687 1029.4843
2571 515.7503 1029.4860
2573 344.4837 1030.4293
2574 516.2860 1030.5574
2575 516.2866 1030.5586
2576 1031.6245 1030.6172
2577 516.3163 1030.6180
2578 516.3167 1030.6188
2579 516.7411 1031.4676
2580 517.2241 1032.4336
2582 518.7546 1035.4946
2583 346.1745 1035.5017
2584 518.7585 1035.5024
2585 519.2468 1036.4790
2586 521.2148 1040.4150
2587 348.1472 1041.4198
2588 521.7609 1041.5072
2589 1042.5192 1041.5119
2590 521.7633 1041.5120
2591 521.7637 1041.5128
2592 521.7637 1041.5128
2596 349.4917 1045.4533
2597 523.7347 1045.4548
2598 349.8477 1046.5213
2599 350.1465 1047.4177
2600 524.7190 1047.4234
2601 524.7720 1047.5294
2602 524.8309 1047.6472
2603 525.2872 1048.5598
2604 350.8097 1049.4073
2606 526.7398 1051.4650
2607 527.3081 1052.6016
2608 527.7291 1053.4436
2609 352.1556 1053.4450
2611 527.8240 1053.6334
2612 353.4999 1057.4779
2613 529.7469 1057.4792
2614 530.2830 1058.5514
2617 532.7045 1063.3944
2618 355.5011 1063.4815
2620 532.7564 1063.4982
2624 356.8172 1067.4298
2625 535.2907 1068.5668
2626 535.3245 1068.6344
2627 535.7132 1069.4118
2628 357.4780 1069.4122
2631 535.8211 1069.6276
2633 537.2868 1072.5590
2634 358.8005 1073.3797
2635 358.8234 1073.4484
2636 538.2986 1074.5826
2637 538.2990 1074.5834
2638 1075.6520 1074.6447
2639 538.3302 1074.6458
2640 538.7232 1075.4318
2641 359.8600 1076.5582
2642 540.1855 1078.3564
2643 360.7964 1079.3674
2644 360.8270 1079.4592
2646 361.8428 1082.5066
2647 542.2815 1082.5484
2649 542.7962 1083.5778
2650 542.7966 1083.5786
2651 542.7985 1083.5824
2653 543.6982 1085.3818
2654 543.7446 1085.4746
2656 364.5236 1090.5490
2657 546.2822 1090.5498
2658 547.2880 1092.5614
2659 547.7343 1093.4540
2660 366.1505 1095.4297
2662 548.8120 1095.6094
2663 548.8126 1095.6106
2664 549.3219 1096.6292
2665 549.8385 1097.6624
2667 551.7271 1101.4396
2668 551.7697 1101.5248
2669 552.2933 1102.5720
2670 368.8125 1103.4157
2671 552.7802 1103.5458
2673 369.8437 1106.5093
2674 557.3037 1112.5928
2675 557.8351 1113.6556
2678 373.4751 1117.4035
2679 559.7101 1117.4056
2680 559.7236 1117.4326
2681 560.3118 1118.6090
2682 560.3128 1118.6110
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