MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2388 464.2687 926.5228
2391 464.7338 927.4530
2392 929.5173 928.5100
2399 310.5118 928.5136
2400 310.5119 928.5139
2402 467.2256 932.4366
2403 467.2417 932.4688
2404 467.2425 932.4704
2405 467.7410 933.4674
2406 312.8032 935.3878
2408 469.2498 936.4850
2409 313.1738 936.4996
2410 313.1739 936.4999
2411 937.7418 936.7345
2412 469.7813 937.5480
2413 470.2148 938.4150
2414 313.8124 938.4154
2415 471.2639 940.5132
2416 314.8531 941.5375
2417 314.8533 941.5381
2420 472.2152 942.4158
2421 943.4495 942.4422
2422 472.2585 942.5024
2423 472.2596 942.5046
2424 943.5708 942.5635
2425 472.2910 942.5674
2427 472.7330 943.4514
2428 472.7369 943.4592
2432 474.2697 946.5248
2433 475.7555 949.4964
2436 952.5576 951.5503
2439 477.2549 952.4952
2440 318.5061 952.4965
2442 318.8783 953.6131
2443 319.1527 954.4363
2444 955.4979 954.4906
2445 319.1768 954.5086
2446 319.1770 954.5092
2448 955.5173 954.5100
2451 319.4901 955.4485
2452 478.7420 955.4694
2453 478.7430 955.4714
2456 321.1691 960.4855
2457 481.7439 961.4732
2458 483.2372 964.4598
2459 965.5547 964.5474
2460 483.2813 964.5480
2461 484.2363 966.4580
2463 323.4878 967.4416
2464 323.8495 968.5267
2465 485.7521 969.4896
2466 485.7532 969.4918
2468 486.2567 970.4988
2469 486.2591 970.5036
2470 324.5087 970.5043
2471 324.8125 971.4157
2472 487.2448 972.4750
2476 487.7687 973.5228
2478 489.2515 976.4884
2480 327.8040 980.3902
2481 491.2652 980.5158
2482 491.7952 981.5758
2484 492.2220 982.4294
2485 492.2591 982.5036
2486 328.5090 982.5052
2487 983.5325 982.5252
2488 328.8135 983.4187
2491 493.2608 984.5070
2492 985.5170 984.5097
2493 985.5175 984.5102
2494 493.2669 984.5192
2495 329.1806 984.5200
2498 494.2725 986.5304
2499 494.2726 986.5306
2500 987.5975 986.5902
2501 494.3033 986.5920
2502 494.3034 986.5922
2511 496.7755 991.5364
2516 497.2360 992.4574
2517 497.2774 992.5402
2519 333.8314 998.4724
2520 500.2447 998.4748
2521 501.2495 1000.4844
2526 502.2656 1002.5166
2528 335.8091 1004.4055
2529 504.2810 1006.5474
2530 336.8281 1007.4625
2531 337.1493 1008.4261
2532 337.1579 1008.4519
2533 505.2951 1008.5756
2537 505.8116 1009.6086
2540 506.7281 1011.4416
2541 338.1547 1011.4423
2542 338.1605 1011.4597
2547 339.1778 1014.5116
2548 508.2693 1014.5240
2549 508.7076 1015.4006
2551 508.7567 1015.4988
2552 508.7570 1015.4994
2553 339.8280 1016.4622
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