MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4429)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2216 732.3314 731.3241
2217 732.3330 731.3257
2219 740.4672 739.4599
2220 371.1263 740.2380
2221 372.1938 742.3730
2223 743.4437 742.4364
2225 372.6891 743.3636
2226 372.6892 743.3638
2227 745.4222 744.4149
2228 373.2153 744.4160
2229 374.1355 746.2564
2230 748.4015 747.3942
2232 377.6846 753.3546
2233 756.3987 755.3914
2234 757.3630 756.3557
2235 379.7309 757.4472
2240 384.2065 766.3984
2241 767.4671 766.4598
2242 385.6449 769.2752
2243 385.6822 769.3498
2244 772.3671 771.3598
2245 386.7210 771.4274
2247 773.4171 772.4098
2252 391.7064 781.3982
2253 782.4066 781.3993
2257 395.2274 788.4402
2258 789.4490 788.4417
2259 789.4515 788.4442
2262 398.2037 794.3928
2263 399.6413 797.2680
2264 400.2169 798.4192
2266 402.2094 802.4042
2267 403.7305 805.4464
2269 405.2264 808.4382
2270 406.2196 810.4246
2271 811.4930 810.4857
2272 406.2502 810.4858
2273 407.2250 812.4354
2278 817.4462 816.4389
2284 412.7362 823.4578
2285 412.7550 823.4954
2286 413.6956 825.3766
2289 415.2331 828.4516
2290 832.4180 831.4107
2291 833.4751 832.4678
2292 417.2417 832.4688
2293 417.2482 832.4818
2294 417.2498 832.4850
2296 836.4631 835.4558
2297 421.7040 841.3934
2299 842.5126 841.5053
2302 423.6545 845.2944
2303 423.7232 845.4318
2304 428.2321 854.4496
2306 855.5191 854.5118
2307 428.2633 854.5120
2310 859.1013 858.0940
2311 863.9138 862.9065
2312 863.9143 862.9070
2313 432.7036 863.3926
2314 432.7572 863.4998
2315 432.7584 863.5022
2316 433.7381 865.4616
2318 435.7760 869.5374
2321 877.4993 876.4920
2322 439.2536 876.4926
2324 440.2336 878.4526
2326 441.2713 880.5280
2327 442.2304 882.4462
2328 884.4596 883.4523
2331 886.4069 885.3996
2332 443.7423 885.4700
2333 444.7208 887.4270
2336 890.5123 889.5050
2342 446.7674 891.5202
2345 447.7672 893.5198
2346 450.2445 898.4744
2347 450.2766 898.5386
2348 899.5461 898.5388
2352 451.7247 901.4348
2355 452.7307 903.4468
2356 453.2510 904.4874
2360 454.2321 906.4496
2362 304.8321 911.4745
2363 456.7446 911.4746
2365 304.8355 911.4847
2367 457.7388 913.4630
2368 457.7487 913.4828
2370 306.4738 916.3996
2373 918.5073 917.5000
2374 459.7580 917.5014
2375 459.7581 917.5016
2376 459.7582 917.5018
2378 921.5273 920.5200
2379 461.2686 920.5226
2380 462.1931 922.3716
2384 309.1493 924.4261
2385 464.1971 926.3796
2386 309.8005 926.3797
2387 464.2442 926.4738
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