MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 2501–2600 (out of 4429)

Page: Previous 1 …21 22 23 24 25 26 27 28 29 30 31 … 45 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2046 308.1667 614.3188
2047 310.6460 619.2774
2048 311.0990 620.1834
2051 312.6780 623.3414
2052 312.7139 623.4132
2053 313.6860 625.3574
2054 315.1697 628.3248
2055 630.3221 629.3148
2062 635.3882 634.3809
2063 319.6854 637.3562
2064 638.3644 637.3571
2065 319.6859 637.3572
2066 641.3397 640.3324
2067 321.2974 640.5802
2068 321.6833 641.3520
2077 322.1646 642.3146
2079 643.3629 642.3556
2084 651.3487 650.3414
2085 326.6756 651.3366
2086 652.4142 651.4069
2087 653.3467 652.3394
2088 654.3104 653.3031
2089 328.1649 654.3152
2090 656.3176 655.3103
2091 328.6625 655.3104
2092 329.1592 656.3038
2093 657.3688 656.3615
2094 329.6699 657.3252
2095 329.6699 657.3252
2096 329.6704 657.3262
2099 659.3904 658.3831
2106 664.4122 663.4049
2114 334.2243 666.4340
2115 335.6811 669.3476
2116 670.3550 669.3477
2118 336.1785 670.3424
2120 672.3471 671.3398
2121 336.6772 671.3398
2122 336.6783 671.3420
2123 336.6889 671.3632
2124 336.6891 671.3636
2125 672.4067 671.3994
2126 337.1825 672.3504
2128 337.2007 672.3868
2129 337.6681 673.3216
2131 676.3424 675.3351
2135 339.1225 676.2304
2136 340.1796 678.3446
2137 679.4144 678.4071
2138 340.6925 679.3704
2139 680.3778 679.3705
2140 340.6934 679.3722
2141 341.6627 681.3108
2142 342.6561 683.2976
2145 343.6670 685.3194
2146 686.3375 685.3302
2147 343.6861 685.3576
2148 343.7011 685.3876
2149 344.1646 686.3146
2150 687.3359 686.3286
2152 688.3438 687.3365
2154 688.8622 687.8549
2155 345.1595 688.3044
2156 346.6730 691.3314
2161 346.7154 691.4162
2162 693.3480 692.3407
2164 696.4055 695.3982
2169 701.3944 700.3871
2170 351.6645 701.3144
2171 351.7007 701.3868
2172 703.2996 702.2923
2173 352.6600 703.3054
2174 704.3416 703.3343
2175 704.3494 703.3421
2176 704.3628 703.3555
2177 352.6960 703.3774
2179 707.4241 706.4168
2180 709.3167 708.3094
2181 355.1863 708.3580
2184 357.6372 713.2598
2185 358.1809 714.3472
2186 358.1812 714.3478
2187 715.3557 714.3484
2188 358.1942 714.3738
2191 718.3182 717.3109
2194 360.1389 718.2632
2195 360.1784 718.3422
2198 360.7069 719.3992
2199 720.4066 719.3993
2201 360.7072 719.3998
2202 362.1914 722.3682
2203 723.4407 722.4334
2204 362.2243 722.4340
2205 363.0164 724.0182
2206 725.3954 724.3881
2207 363.2019 724.3892
2209 727.3757 726.3684
2210 364.1916 726.3686
2212 365.1577 728.3008
2215 365.6463 729.2780
Page: Previous 1 …21 22 23 24 25 26 27 28 29 30 31 … 45 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/