MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 2301–2400 (out of 4429)

Page: Previous 1 …19 20 21 22 23 24 25 26 27 28 29 … 45 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1824 434.0043 432.9970
1825 435.2004 434.1931
1826 435.2011 434.1938
1827 435.2012 434.1939
1828 435.2012 434.1939
1829 435.2012 434.1939
1830 435.2013 434.1940
1831 435.2018 434.1945
1832 435.2019 434.1946
1833 436.0428 435.0355
1834 437.1798 436.1725
1835 437.1809 436.1736
1836 437.1811 436.1738
1837 437.2155 436.2082
1838 437.2157 436.2084
1839 437.2159 436.2086
1840 437.2160 436.2087
1841 437.2162 436.2089
1842 437.2165 436.2092
1843 437.2168 436.2095
1844 437.2169 436.2096
1845 437.2169 436.2096
1846 437.2171 436.2098
1847 437.2174 436.2101
1848 437.2379 436.2306
1849 437.2519 436.2446
1850 437.2521 436.2448
1851 437.2523 436.2450
1852 438.1661 437.1588
1853 438.2531 437.2458
1854 439.1952 438.1879
1855 439.1960 438.1887
1856 439.1967 438.1894
1857 439.1969 438.1896
1858 439.1973 438.1900
1859 439.2134 438.2061
1860 439.2141 438.2068
1861 439.2141 438.2068
1862 439.2141 438.2068
1863 439.2149 438.2076
1864 439.2149 438.2076
1865 439.2151 438.2078
1866 439.2152 438.2079
1867 439.2152 438.2079
1868 439.2153 438.2080
1869 439.2309 438.2236
1870 439.2315 438.2242
1871 439.2317 438.2244
1872 439.2318 438.2245
1873 439.2325 438.2252
1874 439.2325 438.2252
1875 439.2327 438.2254
1876 439.2332 438.2259
1877 441.2307 440.2234
1878 442.2424 441.2351
1879 442.2428 441.2355
1880 443.2466 442.2393
1881 443.2467 442.2394
1882 443.2471 442.2398
1883 444.1100 443.1027
1884 449.2174 448.2101
1885 449.7263 448.7190
1886 451.1957 450.1884
1887 451.1961 450.1888
1888 451.1962 450.1889
1889 453.2100 452.2027
1890 453.2108 452.2035
1891 453.2109 452.2036
1892 453.2114 452.2041
1893 453.2116 452.2043
1894 453.2118 452.2045
1895 453.2119 452.2046
1896 453.2121 452.2048
1897 453.2124 452.2051
1898 453.2478 452.2405
1899 453.3444 452.3371
1900 454.2668 453.2595
1901 455.1605 454.1532
1902 455.1906 454.1833
1903 455.2276 454.2203
1904 455.2282 454.2209
1905 457.1395 456.1322
1906 457.1403 456.1330
1907 457.2775 456.2702
1908 458.2727 457.2654
1909 459.2823 458.2750
1910 461.2748 460.2675
1911 465.2117 464.2044
1912 465.2123 464.2050
1913 467.0645 466.0572
1914 467.0920 466.0847
1915 467.1611 466.1538
1916 467.2263 466.2190
1917 467.2267 466.2194
1918 469.2411 468.2338
1919 469.2414 468.2341
1920 469.2774 468.2701
1921 471.1547 470.1474
1922 471.2686 470.2613
1923 472.2531 471.2458
Page: Previous 1 …19 20 21 22 23 24 25 26 27 28 29 … 45 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/