MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1724 421.2029 420.1956
1725 421.2031 420.1958
1726 421.2037 420.1964
1727 421.2039 420.1966
1728 421.2044 420.1971
1729 421.2044 420.1971
1730 421.2044 420.1971
1731 421.2045 420.1972
1732 421.2055 420.1982
1733 421.2212 420.2139
1734 421.2213 420.2140
1735 421.2219 420.2146
1736 421.2219 420.2146
1737 421.2221 420.2148
1738 421.2224 420.2151
1739 421.2225 420.2152
1740 421.2227 420.2154
1741 421.2228 420.2155
1742 423.1358 422.1285
1743 423.1637 422.1564
1744 423.1640 422.1567
1745 423.1650 422.1577
1746 423.1651 422.1578
1747 423.1994 422.1921
1748 423.1999 422.1926
1749 423.1999 422.1926
1750 423.2000 422.1927
1751 423.2001 422.1928
1752 423.2003 422.1930
1753 423.2004 422.1931
1754 423.2004 422.1931
1755 423.2006 422.1933
1756 423.2006 422.1933
1757 423.2006 422.1933
1758 423.2010 422.1937
1759 423.2010 422.1937
1760 423.2010 422.1937
1761 423.2011 422.1938
1762 423.2012 422.1939
1763 423.2012 422.1939
1764 423.2018 422.1945
1765 423.2019 422.1946
1766 423.2020 422.1947
1767 423.2186 422.2113
1768 423.2186 422.2113
1769 423.2191 422.2118
1770 423.2194 422.2121
1771 423.2198 422.2125
1772 423.2198 422.2125
1773 423.2199 422.2126
1774 423.2201 422.2128
1775 423.2201 422.2128
1776 423.2201 422.2128
1777 423.2202 422.2129
1778 423.2203 422.2130
1779 423.2261 422.2188
1780 423.2262 422.2189
1781 424.0497 423.0424
1782 425.1512 424.1439
1783 425.1797 424.1724
1784 425.1797 424.1724
1785 425.1800 424.1727
1786 425.1801 424.1728
1787 425.1802 424.1729
1788 425.1803 424.1730
1789 425.1806 424.1733
1790 425.2152 424.2079
1791 425.2161 424.2088
1792 425.2162 424.2089
1793 425.2163 424.2090
1794 425.2164 424.2091
1795 425.2165 424.2092
1796 425.2167 424.2094
1797 425.2168 424.2095
1798 425.2168 424.2095
1799 425.2170 424.2097
1800 425.2173 424.2100
1801 425.2174 424.2101
1802 425.2356 424.2283
1803 425.2356 424.2283
1804 425.2357 424.2284
1805 425.2357 424.2284
1806 425.2358 424.2285
1807 425.2358 424.2285
1808 426.0650 425.0577
1809 427.0929 426.0856
1810 427.1291 426.1218
1811 427.1292 426.1219
1812 427.1668 426.1595
1813 427.1956 426.1883
1814 427.1958 426.1885
1815 427.1960 426.1887
1816 427.2310 426.2237
1817 427.2562 426.2489
1818 429.1083 428.1010
1819 429.1458 428.1385
1820 429.1814 428.1741
1821 429.2108 428.2035
1822 432.2822 431.2749
1823 433.1854 432.1781
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