MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1424 387.0975 386.0902
1425 387.1356 386.1283
1426 387.2000 386.1927
1427 387.2000 386.1927
1428 387.2001 386.1928
1429 387.2005 386.1932
1430 387.2011 386.1938
1431 387.2012 386.1939
1432 387.2014 386.1941
1433 387.2042 386.1969
1434 388.2314 387.2241
1435 389.0521 388.0448
1436 389.1595 388.1522
1437 389.1962 388.1889
1438 391.1393 390.1320
1439 391.1741 390.1668
1440 391.1742 390.1669
1441 391.1744 390.1671
1442 391.1745 390.1672
1443 391.1751 390.1678
1444 391.1752 390.1679
1445 391.1752 390.1679
1446 391.1754 390.1681
1447 391.2110 390.2037
1448 391.2117 390.2044
1449 391.2118 390.2045
1450 391.2118 390.2045
1451 391.2119 390.2046
1452 391.2862 390.2789
1453 391.2863 390.2790
1454 393.1519 392.1446
1455 393.1524 392.1451
1456 393.1526 392.1453
1457 393.1526 392.1453
1458 393.1530 392.1457
1459 393.1531 392.1458
1460 393.1532 392.1459
1461 393.1537 392.1464
1462 393.1543 392.1470
1463 393.1545 392.1472
1464 393.1545 392.1472
1465 393.1892 392.1819
1466 393.1895 392.1822
1467 393.1895 392.1822
1468 393.1897 392.1824
1469 393.1902 392.1829
1470 393.1902 392.1829
1471 393.1902 392.1829
1472 393.1903 392.1830
1473 393.1904 392.1831
1474 393.1906 392.1833
1475 393.1906 392.1833
1476 393.1909 392.1836
1477 393.1911 392.1838
1478 393.2098 392.2025
1479 393.2981 392.2908
1480 393.2982 392.2909
1481 393.2983 392.2910
1482 393.2983 392.2910
1483 393.2983 392.2910
1484 393.2984 392.2911
1485 393.2988 392.2915
1486 393.2990 392.2917
1487 393.2991 392.2918
1488 393.2991 392.2918
1489 393.2992 392.2919
1490 393.2992 392.2919
1491 393.2993 392.2920
1492 393.2993 392.2920
1493 393.2994 392.2921
1494 393.2994 392.2921
1495 393.2995 392.2922
1496 393.2995 392.2922
1497 393.2995 392.2922
1498 393.2995 392.2922
1499 393.2996 392.2923
1500 393.2996 392.2923
1501 393.2996 392.2923
1502 393.2996 392.2923
1503 393.2997 392.2924
1504 393.2998 392.2925
1505 393.2999 392.2926
1506 393.2999 392.2926
1507 393.3000 392.2927
1508 393.3000 392.2927
1509 393.3001 392.2928
1510 393.3001 392.2928
1511 393.3002 392.2929
1512 393.3002 392.2929
1513 395.0776 394.0703
1514 395.1683 394.1610
1515 395.1687 394.1614
1516 395.1689 394.1616
1517 395.1689 394.1616
1518 395.1690 394.1617
1519 395.1690 394.1617
1520 395.1690 394.1617
1521 395.1692 394.1619
1522 395.1693 394.1620
1523 395.1696 394.1623
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