MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1324 379.1745 378.1672
1325 379.1745 378.1672
1326 379.1746 378.1673
1327 379.1747 378.1674
1328 379.1747 378.1674
1329 379.1748 378.1675
1330 379.1748 378.1675
1331 379.1752 378.1679
1332 379.1753 378.1680
1333 379.1753 378.1680
1334 379.2112 378.2039
1335 379.2112 378.2039
1336 379.2119 378.2046
1337 381.0268 380.0195
1338 381.1523 380.1450
1339 381.1532 380.1459
1340 381.1532 380.1459
1341 381.1533 380.1460
1342 381.1535 380.1462
1343 381.1537 380.1464
1344 381.1537 380.1464
1345 381.1538 380.1465
1346 381.1541 380.1468
1347 381.1542 380.1469
1348 381.1547 380.1474
1349 381.1547 380.1474
1350 381.1891 380.1818
1351 381.1891 380.1818
1352 381.1891 380.1818
1353 381.1892 380.1819
1354 381.1892 380.1819
1355 381.1893 380.1820
1356 381.1893 380.1820
1357 381.1894 380.1821
1358 381.1897 380.1824
1359 381.1898 380.1825
1360 381.1901 380.1828
1361 381.1901 380.1828
1362 381.1901 380.1828
1363 381.1902 380.1829
1364 381.1902 380.1829
1365 381.1904 380.1831
1366 381.1908 380.1835
1367 381.1909 380.1836
1368 381.1909 380.1836
1369 381.1910 380.1837
1370 381.1911 380.1838
1371 381.1913 380.1840
1372 383.0339 382.0266
1373 383.1032 382.0959
1374 383.1335 382.1262
1375 383.1336 382.1263
1376 383.1378 382.1305
1377 383.1682 382.1609
1378 383.1684 382.1611
1379 383.1685 382.1612
1380 383.1685 382.1612
1381 383.1688 382.1615
1382 383.1689 382.1616
1383 383.1692 382.1619
1384 383.1693 382.1620
1385 383.1693 382.1620
1386 383.1694 382.1621
1387 383.1695 382.1622
1388 383.1697 382.1624
1389 383.1700 382.1627
1390 383.1705 382.1632
1391 383.2046 382.1973
1392 383.2051 382.1978
1393 383.2060 382.1987
1394 383.2062 382.1989
1395 383.2063 382.1990
1396 383.2063 382.1990
1397 383.2065 382.1992
1398 383.2067 382.1994
1399 383.2067 382.1994
1400 383.2068 382.1995
1401 383.2068 382.1995
1402 383.2068 382.1995
1403 384.0514 383.0441
1404 385.0215 384.0142
1405 385.0826 384.0753
1406 385.1186 384.1113
1407 385.1198 384.1125
1408 385.1360 384.1287
1409 385.1845 384.1772
1410 385.1848 384.1775
1411 385.1853 384.1780
1412 385.1854 384.1781
1413 385.1855 384.1782
1414 385.1857 384.1784
1415 385.1860 384.1787
1416 385.1860 384.1787
1417 385.2215 384.2142
1418 385.2450 384.2377
1419 385.2462 384.2389
1420 385.2572 384.2499
1421 385.2574 384.2501
1422 385.2577 384.2504
1423 386.2407 385.2334
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