MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1224 369.2274 368.2201
1225 369.2477 368.2404
1226 369.2479 368.2406
1227 369.2862 368.2789
1228 370.1584 369.1511
1229 371.0438 370.0365
1230 371.1025 370.0952
1231 371.1030 370.0957
1232 371.1033 370.0960
1233 371.1387 370.1314
1234 371.3160 370.3087
1235 371.3161 370.3088
1236 371.3161 370.3088
1237 371.3162 370.3089
1238 371.3164 370.3091
1239 371.3165 370.3092
1240 371.3167 370.3094
1241 371.3169 370.3096
1242 371.3169 370.3096
1243 371.3169 370.3096
1244 371.3171 370.3098
1245 371.3171 370.3098
1246 371.3171 370.3098
1247 371.3171 370.3098
1248 371.3171 370.3098
1249 371.3172 370.3099
1250 371.3173 370.3100
1251 371.3174 370.3101
1252 371.3174 370.3101
1253 371.3174 370.3101
1254 371.3175 370.3102
1255 371.3175 370.3102
1256 371.3175 370.3102
1257 371.3175 370.3102
1258 371.3177 370.3104
1259 371.3178 370.3105
1260 371.3178 370.3105
1261 371.3178 370.3105
1262 371.3178 370.3105
1263 371.3180 370.3107
1264 371.3180 370.3107
1265 371.3181 370.3108
1266 371.3181 370.3108
1267 371.3181 370.3108
1268 372.0523 371.0450
1269 372.1937 371.1864
1270 373.2213 372.2140
1271 375.1427 374.1354
1272 375.1431 374.1358
1273 375.1435 374.1362
1274 375.1805 374.1732
1275 375.1998 374.1925
1276 375.2000 374.1927
1277 375.2004 374.1931
1278 375.2354 374.2281
1279 376.2338 375.2265
1280 376.2347 375.2274
1281 377.0317 376.0244
1282 377.0456 376.0383
1283 377.1203 376.1130
1284 377.1578 376.1505
1285 377.1579 376.1506
1286 377.1579 376.1506
1287 377.1582 376.1509
1288 377.1583 376.1510
1289 377.1585 376.1512
1290 377.1587 376.1514
1291 377.1589 376.1516
1292 377.1591 376.1518
1293 377.1593 376.1520
1294 377.1593 376.1520
1295 377.1598 376.1525
1296 377.1599 376.1526
1297 377.1799 376.1726
1298 377.1954 376.1881
1299 377.1955 376.1882
1300 377.1955 376.1882
1301 377.1955 376.1882
1302 377.1959 376.1886
1303 377.1960 376.1887
1304 377.1961 376.1888
1305 377.1961 376.1888
1306 377.2156 376.2083
1307 379.0018 377.9945
1308 379.1369 378.1296
1309 379.1374 378.1301
1310 379.1380 378.1307
1311 379.1380 378.1307
1312 379.1381 378.1308
1313 379.1388 378.1315
1314 379.1391 378.1318
1315 379.1440 378.1367
1316 379.1729 378.1656
1317 379.1731 378.1658
1318 379.1737 378.1664
1319 379.1742 378.1669
1320 379.1743 378.1670
1321 379.1743 378.1670
1322 379.1744 378.1671
1323 379.1744 378.1671
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