MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1024 357.1248 356.1175
1025 357.1332 356.1259
1026 357.1525 356.1452
1027 357.1529 356.1456
1028 357.1531 356.1458
1029 357.1537 356.1464
1030 357.1537 356.1464
1031 357.1541 356.1468
1032 357.1891 356.1818
1033 357.1903 356.1830
1034 357.9539 356.9466
1035 358.2242 357.2169
1036 359.0207 358.0134
1037 359.1115 358.1042
1038 359.1115 358.1042
1039 359.1122 358.1049
1040 359.1476 358.1403
1041 359.1484 358.1411
1042 359.1487 358.1414
1043 359.2046 358.1973
1044 359.2059 358.1986
1045 359.2405 358.2332
1046 359.2417 358.2344
1047 360.1944 359.1871
1048 360.2397 359.2324
1049 361.0570 360.0497
1050 361.1269 360.1196
1051 361.1270 360.1197
1052 361.1272 360.1199
1053 361.1626 360.1553
1054 361.1633 360.1560
1055 361.1633 360.1560
1056 361.1633 360.1560
1057 361.1636 360.1563
1058 361.1636 360.1563
1059 361.1640 360.1567
1060 361.1642 360.1569
1061 361.1643 360.1570
1062 361.1644 360.1571
1063 361.1644 360.1571
1064 361.2007 360.1934
1065 361.2007 360.1934
1066 361.2008 360.1935
1067 361.2448 360.2375
1068 361.2466 360.2393
1069 363.0364 362.0291
1070 363.0525 362.0452
1071 363.1046 362.0973
1072 363.1418 362.1345
1073 363.1423 362.1350
1074 363.1425 362.1352
1075 363.1427 362.1354
1076 363.1428 362.1355
1077 363.1428 362.1355
1078 363.1431 362.1358
1079 363.1431 362.1358
1080 363.1432 362.1359
1081 363.1433 362.1360
1082 363.1433 362.1360
1083 363.1436 362.1363
1084 363.1776 362.1703
1085 363.1783 362.1710
1086 363.1785 362.1712
1087 363.1785 362.1712
1088 363.1789 362.1716
1089 363.1790 362.1717
1090 363.1793 362.1720
1091 363.1794 362.1721
1092 363.1795 362.1722
1093 363.1796 362.1723
1094 363.1796 362.1723
1095 363.1796 362.1723
1096 363.1799 362.1726
1097 363.1799 362.1726
1098 363.1800 362.1727
1099 363.1800 362.1727
1100 363.1801 362.1728
1101 363.1801 362.1728
1102 363.1801 362.1728
1103 363.1801 362.1728
1104 363.1801 362.1728
1105 363.1803 362.1730
1106 363.2006 362.1933
1107 364.2341 363.2268
1108 364.2348 363.2275
1109 364.2348 363.2275
1110 365.0323 364.0250
1111 365.0518 364.0445
1112 365.1214 364.1141
1113 365.1219 364.1146
1114 365.1224 364.1151
1115 365.1577 364.1504
1116 365.1578 364.1505
1117 365.1579 364.1506
1118 365.1579 364.1506
1119 365.1581 364.1508
1120 365.1581 364.1508
1121 365.1582 364.1509
1122 365.1586 364.1513
1123 365.1586 364.1513
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