MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
824 347.1478 346.1405
825 347.1481 346.1408
826 347.1482 346.1409
827 347.1483 346.1410
828 347.1484 346.1411
829 347.1487 346.1414
830 347.1487 346.1414
831 347.1830 346.1757
832 347.1835 346.1762
833 347.1836 346.1763
834 347.1837 346.1764
835 347.1840 346.1767
836 347.1841 346.1768
837 347.1842 346.1769
838 347.1842 346.1769
839 347.1844 346.1771
840 347.1846 346.1773
841 347.1847 346.1774
842 347.1849 346.1776
843 347.1849 346.1776
844 347.1849 346.1776
845 347.1849 346.1776
846 347.1850 346.1777
847 347.1850 346.1777
848 347.1851 346.1778
849 347.1853 346.1780
850 347.2303 346.2230
851 348.2029 347.1956
852 348.6785 347.6712
853 349.0309 348.0236
854 349.1257 348.1184
855 349.1264 348.1191
856 349.1266 348.1193
857 349.1267 348.1194
858 349.1267 348.1194
859 349.1273 348.1200
860 349.1274 348.1201
861 349.1275 348.1202
862 349.1275 348.1202
863 349.1628 348.1555
864 349.1628 348.1555
865 349.1629 348.1556
866 349.1629 348.1556
867 349.1630 348.1557
868 349.1631 348.1558
869 349.1634 348.1561
870 349.1635 348.1562
871 349.1636 348.1563
872 349.1636 348.1563
873 349.1638 348.1565
874 349.1639 348.1566
875 349.1640 348.1567
876 349.1640 348.1567
877 349.1640 348.1567
878 349.1641 348.1568
879 349.1643 348.1570
880 349.1644 348.1571
881 349.1644 348.1571
882 349.1645 348.1572
883 349.1848 348.1775
884 349.1992 348.1919
885 349.1992 348.1919
886 349.1994 348.1921
887 349.1995 348.1922
888 349.1995 348.1922
889 349.1996 348.1923
890 349.1997 348.1924
891 349.2001 348.1928
892 349.2003 348.1930
893 349.2003 348.1930
894 349.2003 348.1930
895 349.2005 348.1932
896 349.2005 348.1932
897 349.2006 348.1933
898 349.2007 348.1934
899 349.2007 348.1934
900 349.2007 348.1934
901 349.2007 348.1934
902 349.2008 348.1935
903 349.2010 348.1937
904 349.2206 348.2133
905 349.2209 348.2136
906 349.2209 348.2136
907 349.2217 348.2144
908 351.0766 350.0693
909 351.1064 350.0991
910 351.1415 350.1342
911 351.1418 350.1345
912 351.1419 350.1346
913 351.1424 350.1351
914 351.1429 350.1356
915 351.1429 350.1356
916 351.1429 350.1356
917 351.1430 350.1357
918 351.1430 350.1357
919 351.1432 350.1359
920 351.1433 350.1360
921 351.1784 350.1711
922 351.1784 350.1711
923 351.1785 350.1712
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