MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
724 338.1830 337.1757
725 338.1834 337.1761
726 339.0756 338.0683
727 339.0762 338.0689
728 339.0769 338.0696
729 339.1045 338.0972
730 339.1053 338.0980
731 339.1055 338.0982
732 339.1060 338.0987
733 339.1081 338.1008
734 339.1131 338.1058
735 339.1133 338.1060
736 339.1216 338.1143
737 339.1217 338.1144
738 339.1301 338.1228
739 339.1416 338.1343
740 339.1418 338.1345
741 339.1418 338.1345
742 339.1420 338.1347
743 339.1422 338.1349
744 339.1423 338.1350
745 339.1424 338.1351
746 339.1424 338.1351
747 339.1427 338.1354
748 339.1428 338.1355
749 339.1429 338.1356
750 339.1429 338.1356
751 339.1429 338.1356
752 339.1430 338.1357
753 339.1431 338.1358
754 339.1432 338.1359
755 339.1435 338.1362
756 339.1435 338.1362
757 339.1786 338.1713
758 339.1788 338.1715
759 339.1790 338.1717
760 339.1792 338.1719
761 339.1793 338.1720
762 339.1799 338.1726
763 339.1985 338.1912
764 339.1985 338.1912
765 341.0912 340.0839
766 341.0915 340.0842
767 341.1096 340.1023
768 341.1099 340.1026
769 341.1212 340.1139
770 341.1214 340.1141
771 341.1215 340.1142
772 341.1216 340.1143
773 341.1216 340.1143
774 341.1224 340.1151
775 341.1233 340.1160
776 341.1576 340.1503
777 341.1576 340.1503
778 341.1577 340.1504
779 341.1578 340.1505
780 341.1579 340.1506
781 341.1580 340.1507
782 341.1580 340.1507
783 341.1580 340.1507
784 341.1580 340.1507
785 341.1586 340.1513
786 341.1587 340.1514
787 341.1588 340.1515
788 341.1588 340.1515
789 341.1594 340.1521
790 341.1954 340.1881
791 341.1955 340.1882
792 341.1956 340.1883
793 341.1958 340.1885
794 341.1960 340.1887
795 341.1966 340.1893
796 342.0067 340.9994
797 342.1923 341.1850
798 343.0697 342.0624
799 343.0716 342.0643
800 343.1079 342.1006
801 343.1738 342.1665
802 343.1741 342.1668
803 344.1985 343.1912
804 344.2081 343.2008
805 344.2083 343.2010
806 344.2086 343.2013
807 345.0859 344.0786
808 345.1312 344.1239
809 345.1313 344.1240
810 345.1320 344.1247
811 345.1322 344.1249
812 345.1327 344.1254
813 345.1695 344.1622
814 346.2231 345.2158
815 346.2240 345.2167
816 346.2240 345.2167
817 346.2241 345.2168
818 347.1118 346.1045
819 347.1471 346.1398
820 347.1472 346.1399
821 347.1472 346.1399
822 347.1475 346.1402
823 347.1477 346.1404
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