MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
524 327.1422 326.1349
525 327.1423 326.1350
526 327.1423 326.1350
527 327.1423 326.1350
528 327.1425 326.1352
529 327.1428 326.1355
530 327.1429 326.1356
531 327.1430 326.1357
532 327.1430 326.1357
533 327.1430 326.1357
534 327.1430 326.1357
535 327.1786 326.1713
536 327.1786 326.1713
537 327.1788 326.1715
538 327.1790 326.1717
539 327.1793 326.1720
540 327.1800 326.1727
541 328.0492 327.0419
542 328.2136 327.2063
543 329.0324 328.0251
544 329.0555 328.0482
545 329.0918 328.0845
546 329.1215 328.1142
547 329.1226 328.1153
548 329.1576 328.1503
549 329.1579 328.1506
550 329.1581 328.1508
551 329.1581 328.1508
552 329.1582 328.1509
553 329.1584 328.1511
554 329.1584 328.1511
555 329.1585 328.1512
556 329.1586 328.1513
557 329.1946 328.1873
558 330.2293 329.2220
559 331.1164 330.1091
560 331.1165 330.1092
561 331.1530 330.1457
562 331.1534 330.1461
563 331.1537 330.1464
564 331.1758 330.1685
565 331.1765 330.1692
566 331.1898 330.1825
567 331.1899 330.1826
568 331.1899 330.1826
569 331.1900 330.1827
570 331.1901 330.1828
571 331.2108 330.2035
572 332.0569 331.0496
573 332.2081 331.2008
574 332.2082 331.2009
575 332.2086 331.2013
576 333.0564 332.0491
577 333.0565 332.0492
578 333.0851 332.0778
579 333.1312 332.1239
580 333.1313 332.1240
581 333.1317 332.1244
582 333.1320 332.1247
583 333.1321 332.1248
584 333.1322 332.1249
585 333.1324 332.1251
586 333.1326 332.1253
587 333.1329 332.1256
588 333.1550 332.1477
589 333.1679 332.1606
590 333.1683 332.1610
591 333.1684 332.1611
592 333.1685 332.1612
593 333.1689 332.1616
594 333.1691 332.1618
595 333.1691 332.1618
596 333.1691 332.1618
597 333.1692 332.1619
598 333.1693 332.1620
599 333.1693 332.1620
600 333.1696 332.1623
601 333.1697 332.1624
602 333.1698 332.1625
603 333.1702 332.1629
604 333.1896 332.1823
605 333.2042 332.1969
606 333.2046 332.1973
607 333.2047 332.1974
608 333.2051 332.1978
609 333.2051 332.1978
610 333.2053 332.1980
611 333.2053 332.1980
612 333.2054 332.1981
613 333.2055 332.1982
614 333.2055 332.1982
615 333.2056 332.1983
616 333.2056 332.1983
617 333.2059 332.1986
618 334.1345 333.1272
619 334.1372 333.1299
620 334.2234 333.2161
621 335.0357 334.0284
622 335.0911 334.0838
623 335.1092 334.1019
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