MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4429)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
424 323.1104 322.1031
425 323.1110 322.1037
426 323.1110 322.1037
427 323.1110 322.1037
428 323.1110 322.1037
429 323.1111 322.1038
430 323.1113 322.1040
431 323.1116 322.1043
432 323.1118 322.1045
433 323.1462 322.1389
434 323.1471 322.1398
435 323.1472 322.1399
436 323.1472 322.1399
437 323.1472 322.1399
438 323.1474 322.1401
439 323.1474 322.1401
440 323.1474 322.1401
441 323.1475 322.1402
442 323.1475 322.1402
443 323.1475 322.1402
444 323.1475 322.1402
445 323.1476 322.1403
446 323.1479 322.1406
447 323.1480 322.1407
448 323.1481 322.1408
449 323.1482 322.1409
450 323.1483 322.1410
451 323.1485 322.1412
452 323.1485 322.1412
453 323.1652 322.1579
454 323.1658 322.1585
455 323.1660 322.1587
456 323.1662 322.1589
457 323.1665 322.1592
458 323.1837 322.1764
459 323.1840 322.1767
460 323.1841 322.1768
461 323.1843 322.1770
462 323.1843 322.1770
463 323.1844 322.1771
464 323.1844 322.1771
465 323.1844 322.1771
466 323.1847 322.1774
467 323.1848 322.1775
468 323.1848 322.1775
469 323.1848 322.1775
470 323.1848 322.1775
471 323.1850 322.1777
472 323.1850 322.1777
473 323.1850 322.1777
474 323.1851 322.1778
475 325.0607 324.0534
476 325.0992 324.0919
477 325.1064 324.0991
478 325.1070 324.0997
479 325.1261 324.1188
480 325.1261 324.1188
481 325.1263 324.1190
482 325.1266 324.1193
483 325.1266 324.1193
484 325.1267 324.1194
485 325.1267 324.1194
486 325.1268 324.1195
487 325.1268 324.1195
488 325.1270 324.1197
489 325.1271 324.1198
490 325.1271 324.1198
491 325.1273 324.1200
492 325.1276 324.1203
493 325.1631 324.1558
494 325.1631 324.1558
495 325.1632 324.1559
496 325.1633 324.1560
497 325.1638 324.1565
498 325.1639 324.1566
499 325.1639 324.1566
500 325.1640 324.1567
501 325.1641 324.1568
502 325.1823 324.1750
503 325.2007 324.1934
504 326.1672 325.1599
505 327.0753 326.0680
506 327.0754 326.0681
507 327.0757 326.0684
508 327.0759 326.0686
509 327.0760 326.0687
510 327.0762 326.0689
511 327.0763 326.0690
512 327.0805 326.0732
513 327.1043 326.0970
514 327.1053 326.0980
515 327.1060 326.0987
516 327.1068 326.0995
517 327.1071 326.0998
518 327.1216 326.1143
519 327.1218 326.1145
520 327.1234 326.1161
521 327.1413 326.1340
522 327.1421 326.1348
523 327.1422 326.1349
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