MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 44)

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+1

Accession Score Description
1 YHBW_ECOLI 340 Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1

+2

Accession Score Description
1 Q9EX54_STRCO 282 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1

-3

Accession Score Description
1 CH60_ECOLI 258 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
Score Mass Matches Sequences emPAI
3.1 CH60_ECOLI 258 57464 20 (16) 20 (16) 1.44
60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

-20 peptide matches (20 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2040   301.6862 601.3578 601.3547 5.17 0 16 0.13 +1Score > 21 indicates identity
Score > 20 indicates homology		 U K.ISNIR.E
2045   307.6981 613.3816 613.3799 2.83 0 22 0.011 +1Score > 15 indicates identity U K.VTLGPK.G
2151   344.2064 686.3982 686.3963 2.86 0 26 0.013 +1Score > 20 indicates identity U R.NVVLDK.S
2196   360.1786 718.3426 718.3398 3.90 0 23 0.016 +1Score > 18 indicates identity U K.APGFGDR.R
2211   364.7443 727.4740 727.4704 4.95 1 9 0.14 +2Score > 13 indicates identity U K.RVVINK.D
2357   453.7355 905.4564 905.4528 4.04 0 11 0.47 +1Score > 20 indicates identity U K.LIAEAMDK.V + Oxidation (M)
2377   461.2576 920.5006 920.4967 4.25 0 27 0.0063 +1Score > 18 indicates identity U K.SFGAPTITK.D
2496   493.2901 984.5656 984.5604 5.34 0 53 1.7e-005 +1Score > 18 indicates identity U R.GVNVLADAVK.V
2539   337.8466 1010.5180 1010.5145 3.44 0 23 0.0099 +1Score > 16 indicates identity U R.VEDALHATR.A
2572   515.7949 1029.5752 1029.5706 4.51 0 52 3.6e-005 +1Score > 20 indicates identity U K.AVTAAVEELK.A
2594   523.2798 1044.5450 1044.5451 -0.069 0 40 0.00053 +1Score > 20 indicates identity U K.ATLEDLGQAK.R
2605   525.7615 1049.5084 1049.5063 2.04 0 23 0.023 +1Score > 19 indicates identity U K.VGAATEVEMK.E + Oxidation (M)
2784   608.3364 1214.6582 1214.6580 0.18 0 42 0.00024 +1Score > 19 indicates identity U R.EMLPVLEAVAK.A + Oxidation (M)
3178   469.2366 1404.6880 1404.6853 1.88 1 8 0.71 +1Score > 19 indicates identity U K.AVAAGMNPMDLKR.G + 2 Oxidation (M)
3487   571.6002 1711.7788 1711.7536 14.7 1 21 0.021 +1Score > 17 indicates identity U R.QQIEEATSDYDREK.L + Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__2.raw

Score > 19 indicates identity
3610   881.4579 1760.9012 1760.8792 12.5 0 39 0.0006 -1Score > 19 indicates identity U K.AIAQVGTISANSDETVGK.L + Deamidated (NQ)
No other peptide matches in query
3740   615.9792 1844.9158 1844.9116 2.27 0 20 0.019 +1Score > 20 indicates identity
Score > 15 indicates homology		 U K.DTTTIIDGVGEEAAIQGR.V
4194   801.7557 2402.2453 2402.2177 11.5 0 39 0.00041 +1Score > 18 indicates identity U K.ANDAAGDGTTTATVLAQAIITEGLK.A + Deamidated (NQ)
4369   908.8441 2723.5105 2723.4779 12.0 0 32 0.00066 +1Score > 13 indicates identity U K.AGKPLLIIAEDVEGEALATLVVNTMR.G + Deamidated (NQ)
4471   810.9393 3239.7281 3239.7005 8.51 1 36 0.00037 +1Score > 15 indicates identity U R.GYLSPYFINKPETGAVELESPFILLADKK.I + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
CH60_OLEAN 29 56893 3.1
60 kDa chaperonin OS=Oleispira antarctica GN=groL PE=3 SV=1

+4

Accession Score Description
1 TRYP_PIG 176 Trypsin OS=Sus scrofa PE=1 SV=1

+5

Accession Score Description
1 CRP_ECOLI 142 cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1

+6

Accession Score Description
1 K2C1_HUMAN 119 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+7

Accession Score Description
1 EFTU1_ECOLI 103 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+8

Accession Score Description
1 RL4_ECOLI 74 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

+9

Accession Score Description
1 ATDA_ECOLI 63 Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2

+10

Accession Score Description
1 ISPA_ECOLI 61 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1
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