MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:08:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RTCR_ECOLI | 26 | Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | VIOA_CHRVO | 25 | Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q92W49_RHIME | 24 | Putative methyltransferase OS=Rhizobium meliloti (strain 1021) GN=SM_b20515 PE=4 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MOAE_ECOLI | 23 | description |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YLIE_ECOLI | 23 | Putative cyclic di-GMP phosphodiesterase YliE OS=Escherichia coli (strain K12) GN=yliE PE=1 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C9_HUMAN | 23 | Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GAUTC_ARATH | 23 | Probable galacturonosyltransferase 12 OS=Arabidopsis thaliana GN=GAUT12 PE=2 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A4U8R3_9BACT | 23 | SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 28.1 | A4U8R3_9BACT |
23 | 362145 | 5 (3) | 4 (2) | 0.02 | |
| SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 | |||||||
5 peptide matches (4 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2000 |
1 |
541.3478 | 540.3405 | 540.3384 | 3.97 | 0 | 16 | 0.025 | 1Score > 13 indicates identity |
U | R.GVIPR.H |
| 2165 |
349.2277 | 696.4408 | 696.4395 | 1.96 | 1 | 20 | 0.01 | 1Score > 13 indicates identity |
U | R.RGVIPR.H | |
| 2168 |
350.7223 | 699.4300 | 699.4279 | 3.07 | 0 | 12 | 0.16 | 3Score > 16 indicates identity |
U | R.VIEALR.K | |
| 3975 |
1005.0197 | 2008.0248 | 2008.0564 | -15.7 | 0 | 1 | 3.1 | 5Score > 19 indicates identity |
U | R.GGLIGALPGDGAMAAVFAPAPR.V |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| Q70HZ8_9ACTN |
16 | 0 | 28.1 | ||
| description | |||||
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_ECOLI | 21 | DNA-directed RNA polymerase subunit beta' OS=Escherichia coli (strain K12) GN=rpoC PE=1 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | INTF_ECOLI | 21 | Putative prophage CP4-6 integrase OS=Escherichia coli (strain K12) GN=intF PE=3 SV=1 |
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