MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:08:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YHBW_ECOLI | 340 | Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9EX54_STRCO | 282 | Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 2.1 | Q9EX54_STRCO |
282 | 42499 | 20 (16) | 4 (4) | 0.69 | |
| Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1 | |||||||
20 peptide matches (7 non-duplicate, 13 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2108 |
3 |
333.1804 | 664.3462 | 664.3432 | 4.63 | 0 | 25 | 0.007 | 1Score > 16 indicates identity |
U | R.ELFEK.Y |
| 2112 |
1 |
665.3539 | 664.3466 | 664.3432 | 5.20 | 0 | 36 | 0.00052 | 1Score > 16 indicates identity |
U | R.ELFEK.Y |
| 2255 |
1 |
392.2199 | 782.4252 | 782.4221 | 3.98 | 0 | 29 | 0.0012 | 1Score > 13 indicates identity |
U | R.VHCLVR.G |
| 2337 |
890.5123 | 889.5050 | 889.5022 | 3.23 | 0 | 35 | 0.00074 | 1Score > 17 indicates identity |
U | R.GLPVSVYR.V | |
| 2339 |
4 |
445.7603 | 889.5060 | 889.5022 | 4.38 | 0 | 39 | 0.00028 | 1Score > 16 indicates identity |
U | R.GLPVSVYR.V |
| 2719 |
2 |
581.3249 | 1160.6352 | 1160.6302 | 4.35 | 1 | 51 | 2.8e-005 | 1Score > 18 indicates identity |
U | R.DRGLPVSVYR.V |
D:\Xcalibur\Data\Jennifer\20250522 PRT1249 Qingyun\PRT1249__2.rawScore > 19 indicates identity |
|||||||||||
| 2721 |
2 |
387.8862 | 1160.6368 | 1160.6302 | 5.66 | 1 | 35 | 0.0012 | 1Score > 19 indicates identity |
U | R.DRGLPVSVYR.V |
| No other peptide matches in query | |||||||||||
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| YAHA_ECOLI |
67 | 0 | 2.1 | ||
| description | |||||
| YGGE_ECOLI |
56 | 26619 | 2.1 | ||
| Uncharacterized protein YggE OS=Escherichia coli (strain K12) GN=yggE PE=4 SV=1 | |||||
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 258 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 176 | Trypsin OS=Sus scrofa PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CRP_ECOLI | 142 | cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 119 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 103 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_ECOLI | 74 | 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATDA_ECOLI | 63 | Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPA_ECOLI | 61 | Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1 |
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